Immobilization of oligonucleotides on poly(ethylene glycol) brush-coated Si surfaces

被引：31
|
作者：
Cha, TW
Boiadjiev, V
Lozano, J
Yang, H
Zhu, XY
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[3] MetriGenix Inc, Gaithersburg, MD 20878 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 01期
关键词：
D O I：
10.1016/S0003-2697(02)00399-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a hetero-bifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：27 / 32
页数：6
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