Immobilization of oligonucleotides on poly(ethylene glycol) brush-coated Si surfaces

被引：31
|
作者：
Cha, TW
Boiadjiev, V
Lozano, J
Yang, H
Zhu, XY
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[3] MetriGenix Inc, Gaithersburg, MD 20878 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 01期
关键词：
D O I：
10.1016/S0003-2697(02)00399-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a hetero-bifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：27 / 32
页数：6
相关论文
共 50 条
  • [31] Creating patterned poly(dimethylsiloxane) surfaces with amoxicillin and poly(ethylene glycol)
    Bae, Woo-Sung
    Urban, Marek W.
    LANGMUIR, 2006, 22 (24) : 10277 - 10283
  • [32] Stable and functional immobilization of histidine-tagged proteins via multivalent chelator headgroups on a molecular poly(ethylene glycol) brush
    Lata, S
    Piehler, J
    ANALYTICAL CHEMISTRY, 2005, 77 (04) : 1096 - 1105
  • [33] Poly(ethylene glycol)-Coated Magnetite Nanoparticles: Preparation and Characterization
    Amici, Julia
    Celasco, Edvige
    Allia, Paolo
    Tiberto, Paola
    Sangermano, Marco
    MACROMOLECULAR CHEMISTRY AND PHYSICS, 2011, 212 (04) : 411 - 416
  • [34] Poly(ethylene glycol)-coated microfluidic devices for chip electrophoresis
    Schulze, Marcel
    Belder, Detlev
    ELECTROPHORESIS, 2012, 33 (02) : 370 - 378
  • [35] PROTEIN IMMOBILIZATION TO POLYSTYRENE VIA LONG POLY(ETHYLENE GLYCOL) CHAINS
    BERGSTROM, K
    HOLMBERG, K
    BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (08) : 952 - 955
  • [36] Immobilization of Poly(Ethylene Glycol) Terminated with Amine to Titanium Surface by Electrodeposition
    Tanaka, Y.
    Doi, H.
    Iwasaki, Y.
    Yoneyama, T.
    Hanawa, T.
    THERMEC 2006 SUPPLEMENT: 5TH INTERNATIONAL CONFERENCE ON PROCESSING AND MANUFACTURING OF ADVANCED MATERIALS, 2007, 15-17 : 205 - 208
  • [37] Bioapplication of poly(ethylene glycol)-albumin hydrogels: Matrix for enzyme immobilization
    DUrso, EM
    JeanFrancois, J
    Fortier, G
    HYDROGELS AND BIODEGRADABLE POLYMERS FOR BIOAPPLICATIONS, 1996, 627 : 25 - 41
  • [38] Biofunctionalization of metal surface by immobilization of poly(ethylene glycol) terminated amine
    Tanaka, Yuta
    Matsuo, Yuh
    Saito, Haruka
    Tsutsumi, Yusuke
    Doi, Hisashi
    Yoneyama, Takayuki
    Imai, Hachiro
    Hanawa, Takao
    ADVANCED MATERIALS AND PROCESSING, 2007, 26-28 : 765 - +
  • [39] Mixed poly (ethylene glycol) and oligo (ethylene glycol) layers on gold as nonfouling surfaces created by backfilling
    Lokanathan, Arcot R.
    Zhang, Shuai
    Regina, Viduthalai R.
    Cole, Martin A.
    Ogaki, Ryosuke
    Dong, Mingdong
    Besenbacher, Flemming
    Meyer, Rikke L.
    Kingshott, Peter
    BIOINTERPHASES, 2011, 6 (04) : 180 - 188
  • [40] Surface modification of nanoporous alumina surfaces with poly(ethylene glycol)
    Popat, KC
    Mor, G
    Grimes, CA
    Desai, TA
    LANGMUIR, 2004, 20 (19) : 8035 - 8041
12345