Immobilization of oligonucleotides on poly(ethylene glycol) brush-coated Si surfaces

被引：31
|
作者：
Cha, TW
Boiadjiev, V
Lozano, J
Yang, H
Zhu, XY
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[3] MetriGenix Inc, Gaithersburg, MD 20878 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 01期
关键词：
D O I：
10.1016/S0003-2697(02)00399-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a hetero-bifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：27 / 32
页数：6
相关论文
共 50 条
  • [21] Interpenetration and Friction of Brush-Coated Surfaces under External Load: Analytical Theory and SCF Calculations
    Klushin, Leonid I.
    Ivanova, Anna S.
    Polotsky, Alexey A.
    Skvortsov, Alexander M.
    MACROMOLECULES, 2023, 56 (13) : 5188 - 5200
  • [22] Poly[tri(ethylene glycol) ethyl ether methacrylate]-Coated Surfaces for Controlled Fibroblasts Culturing
    Dworak, Andrzej
    Utrata-Wesolek, Alicja
    Szweda, Dawid
    Kowalczuk, Agnieszka
    Trzebicka, Barbara
    AnioL, Jacek
    Sieron, Aleksander L.
    Klama-Baryla, Agnieszka
    Kawecki, Marek
    ACS APPLIED MATERIALS & INTERFACES, 2013, 5 (06) : 2197 - 2207
  • [23] Synthesis and Properties of Magnetite Nanoparticles Coated with Poly(ethylene glycol) and Poly(ethylene imine)
    Zhao, Fangyuan
    Zhang, Baolin
    Wang, Jun
    Tu, Zhijiang
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2013, 13 (10) : 6793 - 6797
  • [24] Poly(ethylene glycol) surface coated magnetic particles
    Flesch, C
    Unterfinger, Y
    Bourgeat-Lami, E
    Duguet, E
    Delaite, C
    Dumas, P
    MACROMOLECULAR RAPID COMMUNICATIONS, 2005, 26 (18) : 1494 - 1498
  • [25] Heparin-coupled poly(poly(ethylene glycol) monomethacrylate)-Si(111) hybrids and their blood compatible surfaces
    Xu, FJ
    Li, YL
    Kang, ET
    Neoh, KG
    BIOMACROMOLECULES, 2005, 6 (03) : 1759 - 1768
  • [26] Ligand Conjugation to Bimodal Poly(ethylene glycol) Brush Layers on Microbubbles
    Chen, Cherry C.
    Borden, Mark A.
    LANGMUIR, 2010, 26 (16) : 13183 - 13194
  • [27] Strong Hydration at the Poly(ethylene glycol) Brush/Albumin Solution Interface
    Zhang, Kexin
    Huang, Hao
    Hung, Hsiang-Chieh
    Leng, Chuan
    Wei, Shuai
    Crisci, Ralph
    Jiang, Shaoyi
    Chen, Zhan
    LANGMUIR, 2020, 36 (08) : 2030 - 2036
  • [28] EXCLUSION OF POLY(ETHYLENE GLYCOL) FROM LIPOSOME SURFACES
    ARNOLD, K
    ZSCHOERNIG, O
    BARTHEL, D
    HEROLD, W
    BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1022 (03) : 303 - 310
  • [29] Mechanical properties of mushroom and brush poly(ethylene glycol)-phospholipid membranes
    Kaufmann, Stefan
    Borisov, Oleg
    Textor, Marcus
    Reimhult, Erik
    SOFT MATTER, 2011, 7 (19) : 9267 - 9275
  • [30] Synthesis and Characterization of Brush Copolymers Based on Methoxy Poly(ethylene glycol) and Poly(ε-caprolactone)
    Bajgai, Madhab Prasad
    Aryal, Santosh
    Parajuli, Darnan Chandra
    Khil, Myung-Seob
    Lee, Duck Rae
    Kim, Hak Yong
    JOURNAL OF APPLIED POLYMER SCIENCE, 2009, 111 (03) : 1540 - 1548
12345