Immobilization of oligonucleotides on poly(ethylene glycol) brush-coated Si surfaces

被引：31
|
作者：
Cha, TW
Boiadjiev, V
Lozano, J
Yang, H
Zhu, XY
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
[3] MetriGenix Inc, Gaithersburg, MD 20878 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 01期
关键词：
D O I：
10.1016/S0003-2697(02)00399-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-density poly(ethylene glycol) (PEG) molecules are grafted onto Si surfaces in a brush-like configuration. We demonstrate that this surface is an excellent substrate for oligonucleotide immobilization. p-Maleimidophenyl isocyanate is used as a hetero-bifunctional cross-linker to tether thiol-modified oligonucleotides to terminal OH groups on the PEG brush. This approach gives excellent immobilization specificity and low background. The immobilized oligonucleotides show high sensitivity for the detection of complementary targets. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：27 / 32
页数：6
相关论文
共 50 条
  • [1] Immobilization of oriented protein molecules on poly(ethylene glycol)-coated Si(111)
    Cha, T
    Guo, A
    Jun, Y
    Pei, DQ
    Zhu, XY
    PROTEOMICS, 2004, 4 (07) : 1965 - 1976
  • [2] Hydrophobic surfaces generated by immobilization of poly(ethylene glycol).
    Cataltarla, E
    McCarthy, TJ
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2003, 226 : U377 - U377
  • [3] A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces
    Piehler, J
    Brecht, A
    Valiokas, R
    Liedberg, B
    Gauglitz, G
    BIOSENSORS & BIOELECTRONICS, 2000, 15 (9-10): : 473 - 481
  • [4] Brush-sheathed particles diffusing at brush-coated surfaces in the thermally responsive PNIPAAm system
    Tu, Huilin
    Hong, Liang
    Anthony, Stephen M.
    Braun, Paul V.
    Granick, Steve
    LANGMUIR, 2007, 23 (05) : 2322 - 2325
  • [5] Force measurements between bacteria and poly(ethylene glycol)-coated surfaces
    Razatos, A
    Ong, YL
    Boulay, F
    Elbert, DL
    Hubbell, JA
    Sharma, MM
    Georgiou, G
    LANGMUIR, 2000, 16 (24) : 9155 - 9158
  • [6] Patterning protein molecules on poly(ethylene glycol) coated Si(111)
    Jun, Y
    Cha, T
    Guo, A
    Zhu, XY
    BIOMATERIALS, 2004, 25 (17) : 3503 - 3509
  • [7] Construction of cell-resistant surfaces by immobilization of poly(ethylene glycol) on gold
    Mougin, K
    Lawrence, MB
    Fernandez, EJ
    Hillier, AC
    LANGMUIR, 2004, 20 (10) : 4302 - 4305
  • [8] Immobilization of oriented protein molecules on poly(ethylene glycol)-coated (Si(111)) (vol 4, pg 1965, 2004)
    Cha, T
    PROTEOMICS, 2004, 4 (09) : 2823 - 2823
  • [9] The role of poly(ethylene glycol) brush architecture in complement activation on targeted microbubble surfaces
    Chen, Cherry C.
    Borden, Mark A.
    BIOMATERIALS, 2011, 32 (27) : 6579 - 6587
  • [10] Immobilization of a functionalized poly(ethylene glycol) onto β-cyclodextrin-coated surfaces by formation of inclusion complexes:: Application to the coupling of proteins
    Guerrouache, M
    Karakasyan, C
    Gaillet, C
    Canva, M
    Millot, MC
    JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 100 (03) : 2362 - 2370
12345