An update on the mouse liver proteome

被引:13
|
作者
Gazzana, Giuseppe [1 ]
Borlak, Juergen [1 ,2 ]
机构
[1] Fraunhofer Inst Toxicol & Expt Med ITEM, Dept Mol Med & Med Biotechnol, Hannover, Germany
[2] Hannover Med Sch, Ctr Pharmacol & Toxicol, D-3000 Hannover, Germany
来源
PROTEOME SCIENCE | 2009年 / 7卷
关键词
TWO-DIMENSIONAL ELECTROPHORESIS; HEPATOCELLULAR-CARCINOMA; 2-DIMENSIONAL DATABASE; SUBSTRATE-SPECIFICITY; GEL-ELECTROPHORESIS; PROTEINS; MEMBRANE; PLASMA; APOPTOSIS; NECROSIS;
D O I
10.1186/1477-5956-7-35
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: Decoding of the liver proteome is subject of intense research, but hampered by methodological constraints. We recently developed an improved protocol for studying rat liver proteins based on 2-DE-MALDI-TOF-MS peptide mass finger printing. This methodology was now applied to develop a mouse liver protein database. Results: Liver proteins were extracted by two different lysis buffers in sequence followed by a liquid-phase IEF pre-fractionation and separation of proteins by 2 DE at two different pH ranges, notably 5-8 and 7-10. Based on 9600 in gel digests a total of 643 mouse liver proteins with high sequence coverage (> 20 peptides per protein) could be identified by MALDI-TOF-MS peptide mass finger printing. Notably, 255 proteins are novel and have not been reported so far by conventional two-dimensional electrophoresis proteome mapping. Additionally, the results of the present findings for mouse liver were compared to published data of the rat proteome to compile as many proteins as possible in a rodent liver database. Conclusion: Based on 2-DE MALDI-TOF-MS a significantly improved proteome map of mouse liver was obtained. We discuss some prominent members of newly identified proteins for a better understanding of liver biology.
引用
收藏
页数:10
相关论文
共 50 条
  • [1] An update on the mouse liver proteome
    Giuseppe Gazzana
    Jürgen Borlak
    Proteome Science, 7
  • [2] Characterization of the scavenger cell proteome in mouse and rat liver
    Paluschinski, Martha
    Jin, Cheng Jun
    Qvartskhava, Natalia
    Goerg, Boris
    Wammers, Marianne
    Lang, Judith
    Lang, Karl
    Poschmann, Gereon
    Stuhler, Kai
    Haeussinger, Dieter
    BIOLOGICAL CHEMISTRY, 2021, 402 (09) : 1073 - 1085
  • [3] Comprehensive and quantitative proteome profiling of the mouse liver and plasma
    Lai, Keane K. Y.
    Kolippakkam, Deepak
    Beretta, Laura
    HEPATOLOGY, 2008, 47 (03) : 1043 - 1051
  • [4] Sample preparation project for the subcellular proteome of mouse liver
    Song, Yanping
    Hao, Yunwei
    Sun, Aihua
    Li, Tao
    Li, Wenrui
    Guo, Lihai
    Yan, Yujuan
    Geng, Chao
    Chen, Ning
    Zhong, Fan
    Wei, Handong
    Jiang, Ying
    He, Fuchu
    PROTEOMICS, 2006, 6 (19) : 5269 - 5277
  • [5] Subcellular proteome of mouse liver; The investigation by combined proteomics approaches
    Zgoda, V.
    Tikhonova, O.
    Viglinskaya, A.
    Archakov, A.
    MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (08) : S96 - S96
  • [6] Evaluation of strategy for analyzing mouse liver plasma membrane proteome
    Ping Chen
    LiJun Zhang
    XuanWen Li
    Xie Wang
    Rui Cao
    Zhen Liu
    JiXian Xiong
    Xia Peng
    YingJuan Wei
    XingFeng Ying
    XianChun Wang
    SongPing Liang
    Science in China Series C: Life Sciences, 2007, 50 : 731 - 738
  • [7] Impact of high-fat diet on the proteome of mouse liver
    Benard, Outhiriaradjou
    Lim, Jihyeon
    Apontes, Pasha
    Jing, Xiaohong
    Angeletti, Ruth H.
    Chi, Yuling
    JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2016, 31 : 10 - 19
  • [8] Evaluation of strategy for analyzing mouse liver plasma membrane proteome
    Ping, Chen
    Lijun, Zhang
    XuanWen, Li
    Xie, Wang
    Rui, Cao
    Zhen, Liu
    JiXian, Xiong
    Xia, Peng
    YingJuan, Wei
    XingFeng, Ying
    XianChun, Wang
    SongPing, Liang
    SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 2007, 50 (06): : 731 - 738
  • [9] Analysis of the mouse liver proteome using advanced mass spectrometry
    Shi, Rong
    Kumar, Chanchal
    Zougman, Alexandre
    Zhang, Yanling
    Podtelejnikov, Alexandre
    Cox, Juegen
    Wisniewski, Jacek R.
    Mann, Matthias
    JOURNAL OF PROTEOME RESEARCH, 2007, 6 (08) : 2963 - 2972