Rapid determination of sildenatil citrate in pharmaceutical preparations using monolithic silica HPLC column

被引：26

作者：

Aboul-Enein, HY ^{[1
]}

Hefnawy, MM ^{[1
]}

机构：

[1] King Faisal Specialist Hosp & Res Ctr, Biol & Med Res Dept MBC03, Pharmaceut Anal Lab, Riyadh 11211, Saudi Arabia

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 17期

关键词：

sildenafil; Viagra; monolithic silica; pharmaceutical analysis; validation;

D O I：

10.1081/JLC-120025052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid, and sensitive high performance liquid chromatography (HPLC) method has been developed and validated for the determination of sildenafil citrate in pharmaceutical preparations. The method was developed utilizing a 100 x 4.6 mm I.D. monolithic silica column and an isocratic elution of 60:40, v/v acetonitrile/water. The elution of the analyte was monitored at 292 nm with a flow rate of 2.0 mL/min. All analyses were conducted at ambient temperature. Linearity was observed in the concentration range from 50-3000 ng/mL, with a correlation coefficient (R-2) greater than 0.999. The limit of detection was 25 ng/mL. Parameters of validation prove the precision of the method and its applicability for the determination of sildenafil citrate in pharmaceutical tablet formulations. The method is fast (less than 1 min) and is suitable for high throughput analysis of the drug.

引用

页码：2897 / 2908

页数：12

共 19 条

[1] High-throughput selected reaction monitoring liquid chromatography-mass spectrometry determination of methylphenidate and its major metabolite, ritalinic acid, in rat plasma employing monolithic columns [J].