Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure

被引:76
作者
Anjum, Fraz [1 ,3 ]
Agabalyan, Natacha A. [2 ]
Sparks, Holly D. [2 ]
Rosin, Nicole L. [2 ]
Kallos, Michael S. [1 ,3 ]
Biernaskie, Jeff [2 ,4 ,5 ,6 ]
机构
[1] Univ Calgary, Pharmaceut Prod Res Facil, 2500 Univ Dr NW, Calgary, AB T2N 4N1, Canada
[2] Univ Calgary, Fac Vet Med, Dept Comparat Biol & Expt Med, 3300 Hosp Dr NW, Calgary, AB T2N 4N1, Canada
[3] Univ Calgary, Schulich Sch Engn, Dept Chem & Petr Engn, 2500 Univ Dr NW, Calgary, AB T2N 4N1, Canada
[4] Univ Calgary, Cumming Sch Med, Dept Surg, 3300 Hosp Dr NW, Calgary, AB T2N 4N1, Canada
[5] Univ Calgary, Alberta Childrens Hosp, Res Inst, 3300 Hosp Dr NW, Calgary, AB TN1 4N1, Canada
[6] Univ Calgary, Hotchkiss Brain Inst, 3300 Hosp Dr NW, Calgary, AB T2N 4N1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
CONTAINING ARTIFICIAL SKIN; MESENCHYMAL STEM-CELLS; GELATIN; SCAFFOLDS; MODEL; GLYCOSAMINOGLYCANS; BIOMATERIALS; REGENERATION; SOLUBILITY; FIBERS;
D O I
10.1038/s41598-017-10735-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cell-based therapies have recently been the focus of much research to enhance skin wound healing. An important challenge will be to develop vehicles for cell delivery that promote survival and uniform distribution of cells across the wound bed. These systems should be stiff enough to facilitate handling, whilst soft enough to limit damage to newly synthesized wound tissue and minimize patient discomfort. Herein, we developed several novel modifiable nanofibre scaffolds comprised of Poly (e-caprolactone) (PCL) and gelatin (GE). We asked whether they could be used as a functional receptacle for adult human Skin-derived Precursor Cells (hSKPs) and how naked scaffolds impact endogenous skin wound healing. PCL and GE were electrospun in a single facile solvent to create composite scaffolds and displayed unique morphological and mechanical properties. After seeding with adult hSKPs, deposition of extracellular matrix proteins and sulphated glycosaminoglycans was found to be enhanced in composite grafts. Moreover, composite scaffolds exhibited significantly higher cell proliferation, greater cell spreading and integration within the nanofiber mats. Transplantation of acellular scaffolds into wounds revealed scaffolds exhibited improvement in dermal-epidermal thickness, axonal density and collagen deposition. These results demonstrate that PCL-based nanofiber scaffolds show promise as a cell delivery system for wound healing.
引用
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页数:17
相关论文
共 64 条
[1]   Animal models in burn research [J].
Abdullahi, A. ;
Amini-Nik, S. ;
Jeschke, M. G. .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2014, 71 (17) :3241-3255
[2]   CELL-MEDIATED DEGRADATION OF TYPE-IV COLLAGEN AND GELATIN FILMS IS DEPENDENT ON THE ACTIVATION OF MATRIX METALLOPROTEINASES [J].
ATKINSON, SJ ;
WARD, RV ;
REYNOLDS, JJ ;
MURPHY, G .
BIOCHEMICAL JOURNAL, 1992, 288 :605-611
[3]   Tissue-engineered skin: Current status in wound healing [J].
Bello Y.M. ;
Falabella A.F. ;
Eaglstein W.H. .
American Journal of Clinical Dermatology, 2001, 2 (5) :305-313
[4]   The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces [J].
Benoit, DSW ;
Anseth, KS .
BIOMATERIALS, 2005, 26 (25) :5209-5220
[5]   SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells [J].
Biernaskie, Jeffrey ;
Paris, Maryline ;
Morozova, Olena ;
Fagan, B. Matthew ;
Marra, Marco ;
Pevny, Larysa ;
Miller, Freda D. .
CELL STEM CELL, 2009, 5 (06) :610-623
[6]  
Chen Ming, 2009, Critical Reviews in Biomedical Engineering, V37, P399, DOI 10.1615/CritRevBiomedEng.v37.i4-5.50
[7]   Functions of hyaluronan in wound repair [J].
Chen, WYJ ;
Abatangelo, G .
WOUND REPAIR AND REGENERATION, 1999, 7 (02) :79-89
[8]   Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge [J].
Choi, YS ;
Hong, SR ;
Lee, YM ;
Song, KW ;
Park, MH ;
Nam, YS .
BIOMATERIALS, 1999, 20 (05) :409-417
[9]   Studies on gelatin-based sponges. Part III: A comparative study of cross-linked gelatin/alginate, gelatin/hyaluronate and chitosan/hyaluronate sponges and their application as a wound dressing in full-thickness skin defect of rat [J].
Choi, YS ;
Lee, SB ;
Hong, SR ;
Lee, YM ;
Song, KW ;
Park, MH .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2001, 12 (01) :67-73
[10]  
Choi YS, 1999, J BIOMED MATER RES, V48, P631, DOI 10.1002/(SICI)1097-4636(1999)48:5<631::AID-JBM6>3.0.CO