Multi-rate nonlinear state and parameter estimation in a bioreactor

This paper concerns real-time, multi-rate, nonlinear, state and parameter estimation in a. pilot-scale, mouse-mouse hybridoma, cell, biochemical reactor. A multi-rate estimator was designed and implemented to estimate specific growth rate and concentrations of viable cells, total cells, glucose, glutamine, and monoclonal antibodies (MAb) in the reactor. These were estimated from frequent measurement (inferred values) of oxygen uptake rate (OUR) and infrequent and delayed measurements of the concentrations of viable cells, total cells, glucose, glutamine, and MAb. The infrequent measurements were available every 2 to 17 hours with time delays of 0.08 to 2.00 hours, and OUR was inferred from dissolved oxygen concentration measurements that were available every 0.17 hour.