In Vitro Study of Hydroxyapatite Coatings on Fibrin Functionalized/Pristine Graphene Oxide for Bone Grafting

被引：34
|
作者：
Deepachitra, Ramani [1 ]
Nigam, Rashi [2 ]
Purohit, Shiv Dutt [3 ]
Kumar, Baskar Santhosh [1 ]
Hemalatha, Thiagarajan [1 ]
Sastry, Thotapalli P. [1 ]
机构：
[1] Cent Leather Res Inst, Bioprod Lab, Chennai 600020, Tamil Nadu, India
[2] VIT Univ, Ctr Biomed Div, Vellore, Tamil Nadu, India
[3] Univ Rajasthan, Ctr Converging Technol, Jaipur 302004, Rajasthan, India
来源：
MATERIALS AND MANUFACTURING PROCESSES | 2015年 / 30卷 / 06期
关键词：
In situ; Hydroxyapatite; Fibrin; Biomedical; Functional; In vitro studies; Graphene oxide; Mechanical strength; Composites; Biomaterials; COMPOSITE SCAFFOLDS; OSTEOGENIC RESPONSE; CALCIUM-PHOSPHATE; CELLS; MINERALIZATION; NANOCOMPOSITES; MIXTURE; GROWTH; FEMUR; ASSAY;
D O I：
10.1080/10426914.2014.994758
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Fibrin functionalized graphene oxide (FGO) and graphene oxide (GO) were used as nucleation sites for the growth of hydroxyapatite (HA). The growth of HA on GO/FGO was done by wet precipitation method, and they were characterized for their physicochemical properties. Cytotoxicity was confirmed by reactive oxygen species assay and cell viability assay; alkaline phosphatase assay, alizarin red test, and protein expression studies showed that FGHA was an excellent composite for osteoblast cell growth and maturation. Alamar blue assay was used to study the enzymatically degraded cytocompatibility of enzymatically degraded components. Results proved that FGHA might be tried as an osteoinductive material, in orthopedic defects.
引用
收藏
页码：804 / 811
页数：8
相关论文
共 50 条
  • [41] Electrophoretic deposition of graphene oxide reinforced hydroxyapatite on the tantalum substrate for bone implant applications: In vitro corrosion and bio-tribological behavior
    Mahmoodi, Mahboobeh
    Hydari, Mohammad Hossein
    Mahmoodi, Leila
    Gazanfari, Lida
    Mirhaj, Marjan
    SURFACE & COATINGS TECHNOLOGY, 2021, 424
  • [42] In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene
    Dosic, Marija
    Erakovic, Sanja
    Jankovic, Ana
    Vukasinovic-Sekulic, Maja
    Matic, Ivana Z.
    Stojanovic, Jovica
    Rhee, Kyong Yop
    Miskovic-Stankovic, Vesna
    Park, Soo-Jin
    JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 2017, 47 : 336 - 347
  • [43] 3D-printed hydroxyapatite/gelatin bone scaffolds reinforced with graphene oxide: Optimized fabrication and mechanical characterization
    Lee, Hoyeol
    Yoo, Jin Myoung
    Ponnusamy, Nandha Kumar
    Nam, Seung Yan
    CERAMICS INTERNATIONAL, 2022, 48 (07) : 10155 - 10163
  • [44] Graphene Oxide/Chitosan/Hydroxyapatite Composite Membranes Enhance Osteoblast Adhesion and Guided Bone Regeneration
    Liu, Sudan
    Li, Zirui
    Wang, Qiuxiang
    Han, Jing
    Wang, Wenying
    Li, Shenghui
    Liu, Huifang
    Guo, Shutao
    Zhang, Jinchao
    Ge, Kun
    Zhou, Guoqiang
    ACS APPLIED BIO MATERIALS, 2021, 4 (11) : 8049 - 8059
  • [45] Facile fabrication of linezolid/strontium coated hydroxyapatite/ graphene oxide nanocomposite for osteoporotic bone defect
    Wu, Shuhui
    Lai, Yunxiao
    Zheng, Xian
    Yang, Yang
    HELIYON, 2024, 10 (11)
  • [46] Graphene Oxide Framework Structures and Coatings: Impact on Cell Adhesion and Pre-Vascularization Processes for Bone Grafts
    Wang, Fanlu
    Saure, Lena Marie
    Schuett, Fabian
    Lorich, Felix
    Rasch, Florian
    Nia, Ali Shaygan
    Feng, Xinliang
    Seekamp, Andreas
    Klueter, Tim
    Naujokat, Hendrik
    Adelung, Rainer
    Fuchs, Sabine
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (06)
  • [47] Induction of Bone Remodeling by Raloxifene-Doped Iron Oxide Functionalized with Hydroxyapatite to Accelerate Fracture Healing
    Wang, Gengqi
    Xu, Wenqiang
    Zhang, Junjie
    Tang, Tian
    Chen, Jing
    Fan, Changchun
    JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2021, 17 (05) : 932 - 941
  • [48] In vitro Studies of Graphene Oxide Reinforced Hydroxyapatite Nanobiocomposite on Human Erythrocytes
    Radha, G.
    Vinod, Rohith K.
    Venkatesan, Balaji
    Vellaichamy, Elangovan
    Balakumar, S.
    61ST DAE-SOLID STATE PHYSICS SYMPOSIUM, 2017, 1832
  • [49] 3D-printed porous calcium silicate scaffolds with hydroxyapatite/graphene oxide hybrid coating for guided bone regeneration
    Liu, Kang
    Jin, Xin
    Wang, Min
    Bai, Yufei
    Jiang, Hongjiang
    Zhu, Qiang
    Zhang, Peng
    CERAMICS INTERNATIONAL, 2025, 51 (01) : 1103 - 1114
  • [50] Bone regeneration based on nano-hydroxyapatite and hydroxyapatite/chitosan nanocomposites: an in vitro and in vivo comparative study
    Tavakol, S.
    Nikpour, M. R.
    Amani, A.
    Soltani, M.
    Rabiee, S. M.
    Rezayat, S. M.
    Chen, P.
    Jahanshahi, M.
    JOURNAL OF NANOPARTICLE RESEARCH, 2013, 15 (01)
12345