In Vitro Study of Hydroxyapatite Coatings on Fibrin Functionalized/Pristine Graphene Oxide for Bone Grafting

被引：35

作者：

Deepachitra, Ramani ^{[1
]}

Nigam, Rashi ^{[2
]}

Purohit, Shiv Dutt ^{[3
]}

Kumar, Baskar Santhosh ^{[1
]}

Hemalatha, Thiagarajan ^{[1
]}

Sastry, Thotapalli P. ^{[1
]}

机构：

[1] Cent Leather Res Inst, Bioprod Lab, Chennai 600020, Tamil Nadu, India

[2] VIT Univ, Ctr Biomed Div, Vellore, Tamil Nadu, India

[3] Univ Rajasthan, Ctr Converging Technol, Jaipur 302004, Rajasthan, India

来源：

MATERIALS AND MANUFACTURING PROCESSES | 2015年 / 30卷 / 06期

关键词：

In situ; Hydroxyapatite; Fibrin; Biomedical; Functional; In vitro studies; Graphene oxide; Mechanical strength; Composites; Biomaterials; COMPOSITE SCAFFOLDS; OSTEOGENIC RESPONSE; CALCIUM-PHOSPHATE; CELLS; MINERALIZATION; NANOCOMPOSITES; MIXTURE; GROWTH; FEMUR; ASSAY;

D O I：

10.1080/10426914.2014.994758

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Fibrin functionalized graphene oxide (FGO) and graphene oxide (GO) were used as nucleation sites for the growth of hydroxyapatite (HA). The growth of HA on GO/FGO was done by wet precipitation method, and they were characterized for their physicochemical properties. Cytotoxicity was confirmed by reactive oxygen species assay and cell viability assay; alkaline phosphatase assay, alizarin red test, and protein expression studies showed that FGHA was an excellent composite for osteoblast cell growth and maturation. Alamar blue assay was used to study the enzymatically degraded cytocompatibility of enzymatically degraded components. Results proved that FGHA might be tried as an osteoinductive material, in orthopedic defects.

引用

页码：804 / 811

页数：8

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