Ex vivo assessment of protective effects of carvacrol against DNA lesions induced in primary rat cells by visible light excited methylene blue (VL plus MB)

被引：18

作者：

Slamenova, D. ^{[1
]}

Horvathova, E. ^{[1
]}

Chalupa, I. ^{[1
]}

Wsolova, L. ^{[2
]}

Navarova, J. ^{[3
]}

机构：

[1] Slovak Acad Sci, Canc Res Inst, Bratislava 83391, Slovakia

[2] Slovak Med Univ, Res Base, Bratislava 83303, Slovakia

[3] Slovak Acad Sci, Inst Expt Pharmacol & Toxicol, Bratislava 84104, Slovakia

来源：

NEOPLASMA | 2011年 / 58卷 / 01期

关键词：

carvacrol; hepatocytes; testicular cells; comet assay; chromosomal aberrations; DPPH assay; ORIGANUM-VULGARE L; ESSENTIAL OILS; IN-VITRO; TESTICULAR CELLS; MAMMALIAN-CELLS; FPG PROTEIN; DAMAGE; THYMOL; ANTIOXIDANTS; HEPATOCYTES;

D O I：

10.4149/neo_2011_01_14

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Carvacrol belongs to frequently occurring phenolic components of essential oils (EOs) and it is present in many kinds of plants. Biological effect of this phenol derivative on human beings is however not sufficiently known. The present study was undertaken to evaluate the level of VL+MB-induced oxidative DNA lesions in hepatocytes and testicular cells (freshly isolated from control or carvacrol-watered rats) by the modified single cell gel electrophoresis (SCGE). The results showed that carvacrol significantly reduced the level of VL+MB-induced oxidized bases (EndoIII- and Fpg-sensitive sites) only in hepatocytes but not in testicular cells. Chromosomal aberration assay of primary hepatocytes, isolated from control or carvacrol-watered rats did not testify any genotoxic activity of carvacrol. We suggest that in vivo applied synthetic carvacrol, whose antioxidative activity was confirmed by DPPH assay, exhibits primarily a strong hepatoprotective activity against oxidative damage to DNA.

引用

页码：14 / 19

页数：6

共 28 条

[1] The effects of thyme volatiles on the induction of DNA damage by the heterocyclic amine IQ and mitomycin C [J].

Aydin, S ;

Basaran, AA ;

Basaran, N .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2005, 581 (1-2) :43-53

[2] The In Vivo Genotoxic Effects of Carvacrol and Thymol in Rat Bone Marrow Cells [J].

Azirak, Sebile ;

Rencuzogullari, Eyyup .

ENVIRONMENTAL TOXICOLOGY, 2008, 23 (06) :728-735

[3] Biological effects of essential oils - A review [J].

Bakkali, F. ;

Averbeck, S. ;

Averbeck, D. ;

Waomar, M. .

FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (02) :446-475

[4] SUBSTRATE-SPECIFICITY OF THE ESCHERICHIA-COLI FPG PROTEIN (FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE) - EXCISION OF PURINE LESIONS IN DNA PRODUCED BY IONIZING-RADIATION OR PHOTOSENSITIZATION [J].

BOITEUX, S ;

GAJEWSKI, E ;

LAVAL, J ;

DIZDAROGLU, M .

BIOCHEMISTRY, 1992, 31 (01) :106-110

[5] DNA SINGLE-STRAND BREAKS, DOUBLE-STRAND BREAKS, AND CROSSLINKS IN RAT TESTICULAR GERM-CELLS - MEASUREMENTS OF THEIR FORMATION AND REPAIR BY ALKALINE AND NEUTRAL FILTER ELUTION [J].

BRADLEY, MO ;

DYSART, G .

CELL BIOLOGY AND TOXICOLOGY, 1985, 1 (03) :181-195

[6] Czech medicinal plants as possible sources of antioxidants [J].

Buricova, Lucie ;

Reblova, Zuzana .

CZECH JOURNAL OF FOOD SCIENCES, 2008, 26 (02) :132-138

[7] Essential oils: their antibacterial properties and potential applications in foods - a review [J].

Burt, S .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 94 (03) :223-253

[8]

Chami N., 2004, Braz J Infect Dis, V8, P217, DOI 10.1590/S1413-86702004000300005

[9] DIRECT ENZYMATIC DETECTION OF ENDOGENOUS OXIDATIVE BASE DAMAGE IN HUMAN LYMPHOCYTE DNA [J].

COLLINS, AR ;

DUTHIE, SJ ;

DOBSON, VL .

CARCINOGENESIS, 1993, 14 (09) :1733-1735

[10] SOME PREVALENT BIOMOLECULES AS DEFENSES AGAINST SINGLET OXYGEN DAMAGE [J].

DAHL, TA ;

MIDDEN, WR ;

HARTMAN, PE .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1988, 47 (03) :357-362

← 1 2 3 →