Both swine and human cells are capable to support the replication of swine hepatitis E virus type 4 in vitro

被引：15

作者：

Zhang, Hou-Yong ^{[1
]}

Chen, Dong-Sheng ^{[1
]}

Wu, Yi-Quan ^{[1
]}

He, Qi-Gai ^{[1
]}

Chen, Huan-Chun ^{[1
]}

Liu, Zheng-Fei ^{[1
]}

机构：

[1] Huazhong Agr Univ, Div Anim Infect Dis, State Key Lab Agr Microbiol, Coll Vet Med, Wuhan 430070, Peoples R China

来源：

VIRUS RESEARCH | 2011年 / 158卷 / 1-2期

关键词：

Swine hepatitis E virus; Replication; Species-barrier; PHYLOGENETIC ANALYSIS; CULTURE-SYSTEM; IDENTIFICATION; INFECTION; SEQUENCES; GENOTYPE; SAMPLES; DEER; HEV;

D O I：

10.1016/j.virusres.2011.03.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using swine anal swab or liver as inocula, cell-culture systems were developed for swine hepatitis E virus (HEV) in swine cells (IBRS-2) and human cells (A549). Both positive and negative strand of swine HEV RNA were detected continuously. Cytopathic effect appeared from passage 8 in IBRS-2 and passage 22 in A549. Viral antigen was detected by indirect immunofluorescent assay in infected cells. Progenies harbored mutations in the third nucleotide of codon. Amino acid changes occurred in passages in IBRS-2 and rescued in passage 10. Full-length genome sequence of a swine HEV isolate from liver was determined to be genotype 4. Taken together, our data suggest that swine HEV is able to replicate in both swine and human cells in vitro. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：289 / 293

页数：5

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