Identification and genomic characterization of grapevine Kizil Sapak virus, a novel grapevine-infecting member of the family Betaflexiviridae

被引：16

作者：

Al Rwahnih, Maher ^{[1
]}

Alabi, Olufemi J. ^{[2
]}

Hwang, Min Sook ^{[1
]}

Stevens, Kristian ^{[1
,3
]}

Golino, Deborah ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

[2] Texas A&M AgriLife Res & Extens Ctr, Dept Plant Pathol & Microbiol, Weslaco, TX 78596 USA

[3] Univ Calif Davis, Dept Evolut & Ecol, Davis, CA 95616 USA

来源：

ARCHIVES OF VIROLOGY | 2019年 / 164卷 / 12期

关键词：

GENERATION;

D O I：

10.1007/s00705-019-04434-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel virus with a (+) single-stranded RNA genome was detected by high-throughput sequencing (HTS) in a sample of grapevine (Vitis vinifera) cv. Kizil Sapak (sample/isolate 127) that originated from Turkmenistan. The complete genome of the virus, tentatively named "grapevine Kizil Sapak virus" (GKSV), is 7,604 nucleotides in length, excluding the poly(A) tail. The genome organization of GKSV, encoded genes, and sequence domains are typical for members of the family Betaflexiviridae, specifically those belonging to the subfamily Trivirinae. Phylogenetic analysis placed GKSV within the subfamily Trivirinae, in the same clade as fig latent virus 1 (FLV-1) but distinct from the clades formed by members of other genera. A comparative analysis of GKSV-127 with the HTS-derived sequences obtained from two additional isolates showed that they are genetic variants of the same virus species. Based on current ICTV species and genus demarcation criteria, and the results of the sequence and phylogenetic analyses, we propose that GKSV and FLV-1 represent a new genus within the subfamily Trivirinae.

引用

页码：3145 / 3149

页数：5

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