CR3 (CD11b/CD18) activation of nasal neutrophils: a measure of upper airway endotoxin exposure

被引：2

作者：

Seth, Romy ^{[1
]}

Romaschin, Alexander D. ^{[2
,3
]}

Ribeiro, Marcos ^{[3
,4
]}

Tarlo, Susan M. ^{[2
,3
]}

机构：

[1] Univ Toronto, Toronto, ON, Canada

[2] St Michaels Hosp, Toronto, ON M5B 1W8, Canada

[3] Toronto Western Hosp, Toronto, ON M5T 2S8, Canada

[4] Univ Sao Paulo, Sch Med, Heart Inst InCor, Div Pulm, Sao Paulo, Brazil

来源：

BIOMARKERS | 2009年 / 14卷 / 07期

关键词：

Chemiluminescence; CR3; endotoxin; lipopolysaccharide; polymorphonuclear leukocytes; LUNG-FUNCTION; BACTERIAL-ENDOTOXIN; AIRBORNE ENDOTOXIN; INHALED ENDOTOXIN; SOLUBLE CD14; DUST; WORKERS; LPS; INHALATION; EXPRESSION;

D O I：

10.3109/13547500903183947

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Inhaled endotoxin (lipopolysaccharide, LPS) initiates an inflammatory response and leads to the expression of CR3 (CD11b/CD18) receptors on polymorphonuclear leukocytes (PMNs). We determined if PMN activation in nasal lavage fluid (NLF) is a possible biomarker of occupational endotoxin exposure. Seven subjects exposed to endotoxin provided NLF samples that were split into three aliquots (negative control - 1 M nicotinamide; sham; positive control - 11 eta g of exogenous LPS) and PMN activation was measured using a chemiluminometer. Differences in mean PMN activation were apparent, negative control: 548 +/- 15.65 RLU 100 mu l(-1); sham: 11469 +/- 2582 RLU 100 mu l(-1); positive control: 42026 +/- 16659 RLU 100 mu l (n = 7; p < 0.05). This technique shows promise as a diagnostic method for measuring upper airway LPS exposure.

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收藏

页码：473 / 479

页数：7

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