Protein-Adsorbed Magnetic-Nanoparticle-Mediated Assay for Rapid Detection of Bacterial Antibiotic Resistance

被引:16
作者
Cowger, Taku A. [1 ,2 ]
Yang, Yaping [3 ]
Rink, David E. [1 ,2 ]
Todd, Trever [1 ,2 ]
Chen, Hongmin [1 ,2 ]
Shen, Ye [4 ]
Yan, Yajun [3 ]
Xie, Jin [1 ,2 ]
机构
[1] Univ Georgia, Dept Chem, Athens, GA 30602 USA
[2] Univ Georgia, Bioimaging Res Ctr, Athens, GA 30602 USA
[3] Univ Georgia, Coll Engn, Athens, GA 30602 USA
[4] Univ Georgia, Dept Epidemiol & Biostat, Athens, GA 30602 USA
基金
美国国家科学基金会;
关键词
ANTIMICROBIAL SUSCEPTIBILITY; SURFACE MODIFICATION; DISK DIFFUSION; AGAR DILUTION; MECHANISM; SEPARATION; COMMUNITY; TESTS;
D O I
10.1021/acs.bioconjchem.7b00016
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Antibiotic susceptibility tests have been used for years as a crucial diagnostic tool against antibiotic-resistant bacteria. However, due to a lack of biomarkers specific to resistant types, these approaches are often time-consuming, inaccurate, and inflexible in drug selections. Here, we present a novel susceptibility test method named protein-adsorbed nanoparticle-mediated matrix-assisted laser desorption-ionization mass spectrometry, or PANMS. Briefly, we adsorb five different proteins (beta-casein, alpha-lactalbumin, human serum albumin, fibrinogen, and avidin) onto the surface of Fe3O4. Upon interaction with bacteria surface, proteins were displaced from the nanoparticle surface, the amounts of which were quantified by matrix-assisted laser desorption ionization mass spectrometry. We find that the protein displacement profile was different distinctive among different bacteria strains and, in particular, between wild-type and drug-resistant strains. More excitingly, we observe bacteria resistant to drugs of the same mechanisms share similar displacement profiles on a linear discriminant analysis (LDA) map. This suggests the possibility of using PANMS to identify the type of mechanism behind antibiotic resistance, which was confirmed in a blind test. Given that PANMS is free of drug incubation and the whole procedure takes less than 50 min, it holds great potential as a high-throughput, low-cost, and accurate drug susceptibility test in the clinic.
引用
收藏
页码:890 / 896
页数:7
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