Absorption spectra of pyrene in bovine serum albumin and caffeine aqueous solutions

被引：9

作者：

Tsukamoto, T ^{[1
]}

Hikida, T ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, DEPT CHEM, MEGURO KU, TOKYO 152, JAPAN

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 1996年 / 95卷 / 03期

关键词：

absorption spectra; pyrene; bovine serum albumin; caffeine; complex;

D O I：

10.1016/1010-6030(95)04268-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interactions between pyrene and bovine serum albumin (BSA) and pyrene and caffeine were studied using absorption spectroscopy in aqueous solutions. In the pyrene-BSA system, an equilibrium between free and adsorbed pyrene was observed in the solution. The absorption spectrum of pyrene adsorbed by BSA was obtained. The equilibrium constant was determined to be 1.1x10(6) dm(3) mol(-1). In the presence of caffeine, the absorption spectrum was a superposition of three species; free pyrene and 1:1 and 1:2 pyrene-caffeine complexes. The equilibrium constants were determined to be 820 and 260 dm(3) mol(-1) for the formation reactions of 1:1 and 1:2 pyrene-caffeine complexes respectively.

引用

页码：271 / 273

页数：3

共 17 条

[1]

BIRKS J B, 1970, P704

[2] LUMINESCENCE PROBE STUDIES OF PYRENE AND 2 CHARGED DERIVATIVES IN NAFION [J].