Fish environmental DNA in Tokyo Bay: A feasibility study on the availability of environmental DNA for fisheries

被引：11

作者：

Hongo, Yuki ^{[1
]}

Nishijima, Shota ^{[1
]}

Kanamori, Yuki ^{[2
]}

Sawayama, Shuhei ^{[1
]}

Yokouchi, Kazuki ^{[1
]}

Kanda, Natsuki ^{[1
]}

Oori, Shiori ^{[1
]}

Uto, Yasuyuki ^{[3
]}

Ishii, Mitsuhiro ^{[4
]}

Mita, Hisanori ^{[5
]}

Akimoto, Seiji ^{[6
]}

Kusano, Akane ^{[7
]}

Okabe, Kyu ^{[6
]}

Yamazaki, Tetsuya ^{[8
]}

Fukuda, Nobuto ^{[1
]}

Onitsuka, Toshihiro ^{[1
]}

Minagawa, Masayuki ^{[1
]}

Okamura, Hiroshi ^{[1
]}

Niwa, Kentaro ^{[9
]}

Nagai, Satoshi ^{[1
]}

Suzuki, Shigenori ^{[1
]}

Yoneda, Michio ^{[10
]}

Yamamoto, Toshihiro ^{[1
]}

Kurogi, Hiroaki ^{[1
]}

机构：

[1] Japan Fisheries Res & Educ Agcy, Fisheries Resources Inst, Kanagawa Ku, 2-12-4 Fukuura, Yokohama, Kanagawa 2368648, Japan

[2] Japan Fisheries Res & Educ Agcy, Hachinohe Field Stn, Fisheries Resources Inst, 25-259 Shimonekurakubo,Samemachi, Hachinohe, Aomori 0310841, Japan

[3] Chiba Prefectural Govt Marine Ind Promot Div, Chuo Ku, 1-1 Ichiba Cho, Chiba 2608667, Japan

[4] Chiba Prefectural Fisheries Res Ctr, Tokyo Bay Fisheries Lab, 3091 Kokubo, Futtsu, Chiba 2930042, Japan

[5] Chiba Prefectural Fisheries Res Ctr, 2492 Hiraiso,Chikuracho, Minamiboso, Chiba 2950024, Japan

[6] Kanagawa Prefectural Fisheries Technol Ctr, Miura, Kanagawa 2380237, Japan

[7] Kanagawa Prefectural Govt, Fisheries Div, Environm & Agr Bur, Naka Ku, 1 Nihon Odori, Yokohama, Kanagawa 2318588, Japan

[8] Hokkaido Res Org, Salmon & Freshwater Fisheries Res Inst, Fisheries Res Dept, 3-373 Kitakashiwagi Cho, Eniwa, Hokkaido 0611433, Japan

[9] Fisheries Agcy, Resources Enhancement Promot Dept, Chiyoda Ku, Tokyo 1008907, Japan

[10] Japan Fisheries Res & Educ Agcy, Hakatajima Field Stn, Fisheries Technol Inst, 2780 Kinoura, Imabari, Ehime 7942305, Japan

来源：

REGIONAL STUDIES IN MARINE SCIENCE | 2021年 / 47卷

关键词：

eDNA; Fish barcoding; eDNA quantity; Fisheries; Monitoring;

D O I：

10.1016/j.rsma.2021.101950

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Environment DNA (eDNA) is a nonlethal sampling method that has potential for evaluating fisheries resources. To evaluate whether eDNA information is useful for fisheries, we collected and analysed eDNA samples from 14 sites in Tokyo Bay from April 2018 to March 2020. Using MiFish universal primers, fish mitochondrial 12S rRNAs were amplified from the eDNA by polymerase chain reaction (PCR) and then sequencing. A total of 535 eDNA samples yielded 44.5 million reads, with 197 of teleosts and 16 of elasmobranchs identified. Of those, 42 identified species were commercially important, with the most abundant being Engraulis japonicus (Japanese anchovy), followed by Konosirus punctatus (dotted gizzard shad) and Lateolabrax japonicusi (Japanese seabass). Mitochondrial cytochrome b copy numbers of K. punctatus and L. japonicus showed a seasonal trend, although catch per unit effort for L. japonicus was not correlated with copy number. Although eDNA is a powerful tool that can detect the presence of fish species from a water sample, caution should be used when interpreting fish biomass based on eDNA dynamics until the method has been better studied. (C) 2021 Elsevier B.V. All rights reserved.

引用

页数：10

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