Analysis of expression rate of cloned beta-lactamase gene in a recombinant of Streptomyces lividans

被引：1
|
作者：
Lee, JH
Lee, KJ
机构：
[1] SEOUL NATL UNIV,DEPT MICROBIOL,SEOUL 151742,SOUTH KOREA
[2] SEOUL NATL UNIV,RES CTR MOL MICROBIOL,SEOUL 151742,SOUTH KOREA
来源：
JOURNAL OF BIOTECHNOLOGY | 1996年 / 52卷 / 02期
关键词：
beta-lactamase; Streptomyces lividans; plasmid copy number; plasmid content; recombinant strain; specific cloned gene expression rate; chemostat;
D O I：
10.1016/S0168-1656(96)01633-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The number and content of the recombinant plasmid (pDML6) in Streptomyces lividans PD6 increased as the specific growth rate decreased. The specific expression rate of the cloned gene, calculated from cultivation in a glucose-limited chemostat, was closely related to the specific growth rate but not to the dosage of cloned gene. (C) 1996 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：161 / 165
页数：5
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