Site-Specific Conjugation of ScFvs Antibodies to Nanoparticles by Bioorthogonal Strain-Promoted Alkyne-Nitrone Cycloaddition

被引：55

作者：

Colombo, Miriam ^{[1
]}

Sommaruga, Silvia ^{[3
]}

Mazzucchelli, Serena ^{[3
]}

Polito, Laura ^{[2
]}

Verderio, Paolo ^{[1
]}

Galeffi, Patrizia ^{[4
]}

Corsi, Fabio ^{[3
]}

Tortora, Paolo ^{[1
]}

Prosperi, Davide ^{[1
,2
]}

机构：

[1] Univ Milano Bicocca, NanoBioLab, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy

[2] CNR, Ist Sci & Tecnol Mol, I-20138 Milan, Italy

[3] Univ Milan, Dipartimento Sci Clin Luigi Sacco, Osped L Sacco, I-20157 Milan, Italy

[4] ENEA, UTAGRI GEN, I-00123 Rome, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 02期

关键词：

bioorthogonal reaction; cell targeting; cycloaddition; nanoparticles; scFv antibody; BREAST-CANCER CELLS; ENGINEERED MAGNETIC NANOPARTICLES; MULTIFUNCTIONAL NANOPARTICLES; CLICK CHEMISTRY; COPPER-FREE; BIOMOLECULES; CYCLOOCTYNES; THERAPY;

D O I：

10.1002/anie.201106775

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

收藏

页码：496 / 499

页数：4

相关论文

共 38 条

[1] The Controlled Display of Biomolecules on Nanoparticles: A Challenge Suited to Bioorthogonal Chemistry [J].

Algar, W. Russ ;

Prasuhn, Duane E. ;

Stewart, Michael H. ;

Jennings, Travis L. ;

Blanco-Canosa, Juan B. ;

Dawson, Philip E. ;

Medintz, Igor L. .

BIOCONJUGATE CHEMISTRY, 2011, 22 (05) :825-858

[2]

[Anonymous], 2006, ANGEW CHEM, DOI DOI 10.1002/ange.200602471

[3] Copper-free click chemistry in living animals [J].

Chang, Pamela V. ;

Prescher, Jennifer A. ;

Sletten, Ellen M. ;

Baskin, Jeremy M. ;

Miller, Isaac A. ;

Agard, Nicholas J. ;

Lo, Anderson ;

Bertozzi, Carolyn R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (05) :1821-1826

[4] A screening paradigm for the design of improved polymer-coated superparamagnetic iron oxide nanoparticles [J].

Chen, Suelin ;

Reynolds, Fred ;

Yu, Lingtau ;

Weissleder, Ralph ;

Josephson, Lee .

JOURNAL OF MATERIALS CHEMISTRY, 2009, 19 (35) :6387-6392

[5] HER2 Expression in Breast Cancer Cells Is Downregulated Upon Active Targeting by Antibody-Engineered Multifunctional Nanoparticles in Mice [J].

Corsi, Fabio ;

Fiandra, Luisa ;

De Palma, Clara ;

Colombo, Miriam ;

Mazzucchelli, Serena ;

Verderio, Paolo ;

Allevi, Raffaele ;

Tosoni, Antonella ;

Nebuloni, Manuela ;

Clementi, Emilio ;

Prosperi, Davide .

ACS NANO, 2011, 5 (08) :6383-6393

[6] Towards Ideal Magnetofluorescent Nanoparticles for Bimodal Detection of Breast-Cancer Cells [J].

Corsi, Fabio ;

De Palma, Clara ;

Colombo, Miriam ;

Allevi, Raffaele ;

Nebuloni, Manuela ;

Ronchi, Silvia ;

Rizzi, Giuseppina ;

Tosoni, Antonella ;

Trabucchi, Emilio ;

Clementi, Emilio ;

Prosperi, Davide .

SMALL, 2009, 5 (22) :2555-2564

[7] Bioconjugation with Strained Alkenes and Alkynes [J].

Debets, Marjoke F. ;

Van Berkel, Sander S. ;

Dommerholt, Jan ;

Dirks, A. J. ;

Rutjes, Floris P. J. T. ;

Van Delft, Floris L. .

ACCOUNTS OF CHEMICAL RESEARCH, 2011, 44 (09) :805-815

[8] Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging [J].

Dieterich, Daniela C. ;

Lee, Jennifer J. ;

Link, A. James ;

Graumann, Johannes ;

Tirrell, David A. ;

Schuman, Erin M. .

NATURE PROTOCOLS, 2007, 2 (03) :532-540

[9] Copper toxicity, oxidative stress, and antioxidant nutrients [J].

Gaetke, LM ;

Chow, CK .

TOXICOLOGY, 2003, 189 (1-2) :147-163

[10]

Haun JB, 2010, NAT NANOTECHNOL, V5, P660, DOI [10.1038/nnano.2010.148, 10.1038/NNANO.2010.148]

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