A Rice Yellow-Green-Leaf 219 Mutant Lacking the Divinyl Reductase Affects Chlorophyll Biosynthesis and Chloroplast Development

被引：5

作者：

Long, Wuhua ^{[1
]}

Long, Sifang ^{[2
]}

Jiang, Xue ^{[1
]}

Xu, Haifeng ^{[1
]}

Peng, Qiang ^{[1
]}

Li, Jiali ^{[1
]}

Zhang, Xichun ^{[1
]}

Zhang, Dashuang ^{[1
]}

Liu, Xuewei ^{[1
]}

Zhu, Susong ^{[1
]}

机构：

[1] Guizhou Acad Agr Sci, Guizhou Rice Res Inst, Guiyang 550006, Peoples R China

[2] Qiannan Med Coll Nationalities, Duyun 558000, Peoples R China

来源：

JOURNAL OF PLANT GROWTH REGULATION | 2022年 / 41卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Oryza sativa yellow-green-leaf mutant; Chloroplast; Chlorophyll; PENTATRICOPEPTIDE REPEAT PROTEIN; HARVESTING COMPLEX-II; POINT MUTATION; COLD STRESS; GENE; DEGRADATION; SUBUNITS; ENCODES; BIOGENESIS; SENESCENCE;

D O I：

10.1007/s00344-021-10508-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Leaf-color mutants are ideal genetic materials for studying the mechanism of chlorophyll (Chl) biosynthesis and chloroplast development. Here we isolated and identified a novel rice yellow-green-leaf mutant from a Co-60-irradiated mutant pool, named ygl219. In the ygl219 mutant, the pigments contents were decreased and the development of chloroplast was arrested. Map-based cloning and subsequent DNA sequencing analysis revealed a single nucleotide substitution happened in divinyl reductase (DVR) gene in the ygl219 mutant. Genetic complementation of the ygl219 mutant restored normal green leaf by expressing full length coding sequence of DVR. Subcellular localization indicated the DVR protein was located in the chloroplast. qRT-PCR analysis revealed that most of the genes associated with chlorophyll biosynthesis were significantly upregulated, while most of the genes associated with chloroplast development and photosynthesis were significantly downregulated in the ygl219 mutant. These data indicated that DVR plays an important role in chlorophyll biosynthesis and chloroplast development in rice.

引用

页码：3233 / 3242

页数：10

共 12 条

[11] The rice white green leaf 2 gene causes defects in chloroplast development and affects the plastid ribosomal protein S9
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RICE, 2018, 11
[12] Yellow-Green Leaf 19 Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (23)

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