Interactions between rare-earth ions and DNA of Bashibai sheep

被引：12

作者：

Aniwashi, J. ^{[1
,2
]}

Kaleri, H. A. ^{[1
,3
]}

Sulaiman, Y. ^{[2
]}

Qi-fa, L. ^{[1
]}

Zhuang, X. ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Peoples R China

[2] Xinjiang Agr Univ, Coll Anim Sci, Urumqi, Peoples R China

[3] Sindh Agr Univ, Dept Anim Breeding & Genet, Fac Anim Husb & Vet Sci, Tandojam, Pakistan

来源：

GENETICS AND MOLECULAR RESEARCH | 2011年 / 10卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Rare earth; Nucleic acid chain; Microcalorimetry; Bashibai sheep; BINDING; LA3+;

D O I：

10.4238/vol10-2gmr1070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between rare-earth ions and DNA from Bashibai sheep was studied by microcalorimetry and electrochemistry. The DNA chain was found to have four to five binding sites for rare-earth ions. The binding affinity was about 10(-5)-10(-6) M. It was also found that smaller ions caused more heat to be released in the process of binding and bound more readily to the nucleic acid chain. This is attributed to the enhanced ability of polarization of smaller ions and reduced steric hindrance compared to larger ions. The electrochemistry results show that rare-earth ions could be inserted into the DNA helix, producing a new complex with electrochemically active groups. The rare-earth ions and DNA complex reached equilibrium after a 90-min incubation at room temperature.

引用

页码：1075 / 1083

页数：9

共 20 条

[1]

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[2] Distribution of some rare earth elements and their binding species with proteins in human liver studied by instrumental neutron activation analysis combined with biochemical techniques [J].