Glial Cell Calcium Signaling Mediates Capillary Regulation of Blood Flow in the Retina

被引：117

作者：

Biesecker, Kyle R. ^{[1
]}

Srienc, Anja I. ^{[1
]}

Shimoda, Angela M. ^{[1
]}

Agarwal, Amit ^{[2
]}

Bergles, Dwight E. ^{[2
]}

Kofuji, Paulo ^{[1
]}

Newman, Eric A. ^{[1
]}

机构：

[1] Univ Minnesota Twin Cities, Dept Neurosci, 6-145 Jackson Hall,321 Church St SE, Minneapolis, MN 55455 USA

[2] Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21205 USA

来源：

JOURNAL OF NEUROSCIENCE | 2016年 / 36卷 / 36期

关键词：

blood flow; calcium signaling; capillary; glia; neurovascular coupling; retina; NITRIC-OXIDE SYNTHASE; CORTEX IN-VIVO; FUNCTIONAL HYPEREMIA; ALZHEIMERS-DISEASE; NEURONAL-ACTIVITY; VASCULAR NETWORK; NORMAL BRAIN; PERICYTES; MOUSE; RESPONSES;

D O I：

10.1523/JNEUROSCI.1782-16.2016

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The brain is critically dependent on the regulation of blood flow to nourish active neurons. One widely held hypothesis of blood flow regulation holds that active neurons stimulate Ca2+ increases in glial cells, triggering glial release of vasodilating agents. This hypothesis has been challenged, as arteriole dilation can occur in the absence of glial Ca2+ signaling. We address this controversy by imaging glial Ca2+ signaling and vessel dilation in the mouse retina. We find that sensory stimulation results in Ca2+ increases in the glial endfeet contacting capillaries, but not arterioles, and that capillary dilations often follow spontaneous Ca2+ signaling. In IP3R2(-/-) mice, where glial Ca2+ signaling is reduced, light-evoked capillary, but not arteriole, dilation is abolished. The results show that, independent of arterioles, capillaries actively dilate and regulate blood flow. Furthermore, the results demonstrate that glial Ca2+ signaling regulates capillary but not arteriole blood flow.

引用

页码：9435 / 9445

页数：11

共 57 条

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