Ultraviolet radiation-induced DNA damage in promoter elements inhibits gene expression

被引：6
作者：
Ghosh, R
Tummala, R
Mitchell, DL
机构：
[1] AMC Canc Res Ctr, Dept Canc Causat & Prevent, Denver, CO 80214 USA
[2] Univ Colorado, Ctr Comprehens Canc, Denver, CO 80214 USA
[3] MD Anderson Canc Ctr, Dept Carcinogenesis, Smithville, TX 78957 USA
来源：
FEBS LETTERS | 2003年 / 554卷 / 03期
关键词：
ultraviolet radiation; DNA damage; gene expression;
D O I：
10.1016/S0014-5793(03)01215-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Repair of DNA damage in gene promoters is slower than in actively transcribed genes. Persistent damage in gene promoters though transient can have significant biological effects on regulated gene expression. In this study we investigated the effect of ultraviolet radiation on gene promoter-associated functions when DNA damage is located within and outside transcription factor binding sites. Our results show that both cyclobutane pyrimidine dimers and (6-4) photoproducts inhibit DNA-protein interaction, in vitro transcript production and transactivation of reporter genes. The biological significance of transient DNA damage as a mechanism in carcinogenesis is discussed. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：427 / 432
页数：6
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