Engineering N-glycosylation pathways in the baculovirus-insect cell system

被引：78
作者：
Jarvis, DL [1 ]
Kawar, ZS [1 ]
Hollister, JR [1 ]
机构：
[1] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 05期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
D O I：
10.1016/S0958-1669(98)80041-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The inability to produce eukaryotic glycoproteins with complex N-linked glycans is a major limitation of the baculovirus-insect cell expression system. Recent studies have demonstrated that metabolic engineering can be used to extend the glycoprotein processing capabilities of lepidopteran insect cells. This approach is being used to develop new baculovirus-insect cell expression systems that can produce more authentic recombinant glycoproteins and obtain new information on insect N-glycosylation pathways.
引用
收藏
页码：528 / 533
页数：6
相关论文
共 57 条
  • [1] INSECT CELLS CONTAIN AN UNUSUAL, MEMBRANE-BOUND BETA-N-ACETYLGLUCOSAMINIDASE PROBABLY INVOLVED IN THE PROCESSING OF PROTEIN N-GLYCANS
    ALTMANN, F
    SCHWIHLA, H
    STAUDACHER, E
    GLOSSL, J
    MARZ, L
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) : 17344 - 17349
  • [2] PROCESSING OF ASPARAGINE-LINKED OLIGOSACCHARIDES IN INSECT CELLS - N-ACETYLGLUCOSAMINYLTRANSFERASE I AND II ACTIVITIES IN CULTURED LEPIDOPTERAN CELLS
    ALTMANN, F
    KORNFELD, G
    DALIK, T
    STAUDACHER, E
    GLOSSL, J
    [J]. GLYCOBIOLOGY, 1993, 3 (06) : 619 - 625
  • [3] PROCESSING OF ASPARAGINE-LINKED OLIGOSACCHARIDES IN INSECT CELLS - EVIDENCE FOR ALPHA-MANNOSIDASE-II
    ALTMANN, F
    MARZ, L
    [J]. GLYCOCONJUGATE JOURNAL, 1995, 12 (02) : 150 - 155
  • [4] [Anonymous], 1995, Glycoproteins
  • [5] PURIFICATION AND CHARACTERIZATION OF A NOVEL BROAD-SPECIFICITY (ALPHA-1-]2, ALPHA-1-]3 AND ALPHA-1-]6) MANNOSIDASE FROM RAT-LIVER
    BONAY, P
    HUGHES, RC
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 197 (01): : 229 - 238
  • [6] ISOLATION AND CHARACTERIZATION OF MOSQUITO CELL-MEMBRANE GLYCOPROTEINS
    BUTTERS, TD
    HUGHES, RC
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 640 (03) : 655 - 671
  • [7] STEPS IN THE BIOSYNTHESIS OF MOSQUITO CELL-MEMBRANE GLYCOPROTEINS AND THE EFFECTS OF TUNICAMYCIN
    BUTTERS, TD
    HUGHES, RC
    VISCHER, P
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 640 (03) : 672 - 686
  • [8] CHEN WY, 1991, J BIOL CHEM, V266, P8192
  • [9] Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis
    Chui, D
    OhEda, M
    Liao, YF
    Panneerselvam, K
    Lal, A
    Marek, KW
    Freeze, HH
    Moremen, KW
    Fukuda, MN
    Marth, JD
    [J]. CELL, 1997, 90 (01) : 157 - 167
  • [10] POSTTRANSLATIONAL MODIFICATIONS OF THE CUTICULAR PROTEINS OF HYALOPHORA-CECROPIA FROM DIFFERENT ANATOMICAL REGIONS AND METAMORPHIC STAGES
    COX, DL
    WILLIS, JH
    [J]. INSECT BIOCHEMISTRY, 1987, 17 (03): : 469 - 484
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