Marker development for the genetic study of natural variation in Arabidopsis thaliana

被引：3
|
作者：
Nemri, Adnane [1 ]
Neff, Michael M.
Burrell, Michael
Jones, Jonathan D. G.
Studholme, David J.
机构：
[1] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[2] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA
来源：
BIOINFORMATICS | 2007年 / 23卷 / 22期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1093/bioinformatics/btm501
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Summary: We report AtPRIMER, an application that automates the discovery of new polymorphic markers between ecotypes of Arabidopsis thaliana. On specifying two ecotypes and the genomic region of interest, the script retrieves all corresponding single nucleotide polymorphisms (SNPs) and generates CAPS and/or dCAPS PCR primer sequences. We show that AtPRIMER accurately found specific polymorphic markers for our linkage mapping project. AtPRIMER will therefore be useful for efficient marker development with high density and specificity. Availability: This PERL/CGI program is available for non-commercial users at http://www.AtPRIMER.tsl.ac.uk. The web-based version is available for public use at the same URL. Contact: adnane.nemri@tsl.ac.uk.
引用
收藏
页码：3108 / 3109
页数：2
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