Effect of Co-Cultured Bone Marrow Mesenchymal Stem Cells (BMSC) and Neuropilin 1 on the Migration of Endometrial Stromal Cells and Epithelial-Mesenchymal Transition in Adenomyosis

被引：0
作者：
Liu, Lixue [1 ]
Wang, Saren [2 ]
Li, Debang [1 ]
Tuo, Ya [3 ]
机构：
[1] Inner Mongolia Med Univ, Master Reprod Med Ctr, Affiliated Hosp, Hohhot 010050, Inner Mongolia, Peoples R China
[2] Inner Mongolia Med Univ, Bachelor Reprod Med Ctr, Affiliated Hosp, Hohhot 010050, Inner Mongolia, Peoples R China
[3] Inner Mongolia Med Univ, Reprod Med Ctr, Affiliated Hosp, Hohhot 010050, Inner Mongolia, Peoples R China
来源：
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2022年 / 12卷 / 10期
关键词：
Neuropilin; 1; Epithelial-Mesenchymal Transition; Bone Marrow Mesenchymal Stem Cells; Co-Culture; Adenomyosis; INVASION; PROLIFERATION;
D O I：
10.1166/jbt.2022.3139
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Our study intends to assess the effect of co-cultured bone marrow mesenchymal stem cells (BMSC) and Neuropilin 1 on the migration of endometrial stromal cells and epithelial-mesenchymal transition in adenomyosis. qRT-PCR was utilized for measuring Neuropilin 1 level in adenomyosis tissues and cells. Endometrial stromal cells were treated with BMSC, si-Neuropilin 1, and pc-DNANeuropilin followed by analysis of cell proliferation and migration, as well as the expression of epithelial-mesenchymal transition (EMT)-related proteins. Neuropilin 1 expression was significantly upregulated in 77 adenomyosis patients. Neuropilin 1 expression showed a positive correlation IP: 182.75.148.10 On: Sat, 20 Aug 2022 10:37:01 with the diagnostic sensitivity of adenomyosis. BMSC + pc-DNA-Neuropilin 1 treatment significantly promoted cell viability, migration, and EMT which were reduced by BMSC+si-Neuropilin 1. In conclusion, our study demonstrates that co-culture of BMSCs and Neuropilin 1 increases cell viability, migration, and EMT in adenomyosis.
引用
收藏
页码：2063 / 2068
页数：6
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