Nocardioides daecheongensis sp nov., isolated from soil

Strain K152-16(T) was isolated from a soil sample collected from Daecheong Island of. Incheon region, South Korea. K152-16(T) was Gram-staining-positive, aerobic, non-spore-forming, nonmotile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16(T) represented a member of the genus Nocardioides, being most closely related to the type strains of species of the genus Nocardioides, Nocardioides maradonensis RP-B30(T) (97.8% sequence similarity) and Nocardioides ultimimeridianus RP-B26(T) (97.0%). The fatty acid profile of K152-16(T) was dominated by C(18:1 omega)9c, C-17:1 omega C, C-16:0, C-18:0 10-methyl (TBSA), C-16:0 2-OH and C-17:0 2-OH. The major isoprenoid quinone was MK8(H4), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3 gamma-type with LL-diaminopimelic acid. The genomic DNA G+C content of KIS2-16(T) was 64.9 molok. Strain KIS2-16(T) showed DNA DNA hybridization values of less than 70% with the closely related species of the genus Nocardioides. Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus Nocardioides, for which the name Nocardioides daecheongensis sp. nov. (type strain KIS2-16(T)= DSM 27136(T)=KACC 17297(T)=NBRC 109597(T)) is proposed.