CHLAMYDIA PNEUMONIAE INFECTION AS A RISK FACTOR FOR ACCELERATED ATHEROSCLEROSIS IN HEMODIALYSIS PATIENTS

被引:0
作者
Schiavoni, G. [1 ]
Di Pietro, M. [1 ]
Ronco, C. [2 ]
de Cal, M. [2 ]
Cazzavillan, S. [3 ]
Rassu, M. [4 ]
Ncoletti, M. [5 ]
del Piano, M. [2 ]
Sessa, R. [1 ]
机构
[1] Univ Roma La Sapienza, Dept Publ Hlth Sci, I-00185 Rome, Italy
[2] San Bortolo Hosp, Dept Nephrol Dialysis & Transplantat, Vicenza, Italy
[3] San Bortolo Hosp, Dept Pathol, Vicenza, Italy
[4] San Bortolo Hosp, Dept Microbiol, Vicenza, Italy
[5] Univ G DAnnunzio, Dept Biomed Sci, Chieti, Italy
关键词
Chlamydia pneumoniae; end-stage renal disease; peripheral blood mononuclear cells; real-time PCR; atherosclerotic cardiovascular disease; inflammatory markers; INTIMA-MEDIA THICKNESS; DIALYSIS PATIENTS; CARDIOVASCULAR RISK; EXPRESSION; DISEASES; PROTEIN; STRESS; UREMIA; INTERLEUKIN-6; ASSOCIATION;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Atherosclerotic cardiovascular disease is the main cause of morbidity and mortality for end-stage renal disease patients undergoing chronic haemodialysis (HD). Several studies in recent years have identified Chlamydia pneumoniae, a respiratory pathogen, as risk factor for cardiovascular diseases in the general population. The aim of our study is to evaluate chlamydial load, in peripheral blood mononuclear cells (PBMC) of HD patients. Furthermore, the correlation between DNA chlamydial load and markers of inflammation was also examined. PBMC specimens isolated from 49 HD patients and 46 blood donors were analyzed for the presence of C. pneumoniae DNA by real-time PCR and ompA nested touchdown PCR. In HD patients, plasma levels of several inflammatory markers were also determined. A significantly higher rate of C. pneumoniae DNA was found in HD patients (44.9%) than in blood donors (19.6%) (p=0.016); HD patients were also more likely to have a significantly high chlamydial load (p=0.0004). HD patients with atherosclerotic cardiovascular diseases have a significantly greater chlamydial load than HD patients without cardiovascular diseases (p= 0.006). A significantly higher value of C-reactive protein, IL-6 and advanced oxidative protein products was found in HD patients with a greater chlamydial load (p<0.05). Likewise, a significantly lower monocyte HLA-DR percentage (p=0.011) as well as a lower monocyte HLA-DR expression were found in such patients (p= 0.007). In conclusion, our results show that HD patients are at high risk of C. pneumoniae infection correlated with chronic inflammatory response which in turn can lead to accelerated atherosclerosis and other long-term clinical complications such as myocardial infarction and stroke.
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页码:367 / 375
页数:9
相关论文
共 37 条
[1]   ACUTE REJECTION FEATURES IN DUAL KIDNEY TRANSPLANT RECIPIENTS FROM ELDERLY DONORS: COMPARISON OF CALCINEURIN INHIBITOR-BASED AND CALCINEURIN INHIBITOR-FREE IMMUNOSUPPRESSIVE PROTOCOLS [J].
Aiello, F. B. ;
Furian, L. ;
Marino, S. ;
Marchini, F. ;
Cardillo, M. ;
De Fazio, N. ;
Rigotti, P. ;
Valente, M. .
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2009, 22 (04) :1001-1007
[2]   The paradox of the "body mass index paradox" in dialysis patients: associations of adiposity with inflammation [J].
Beddhu, S ;
Ramkumar, N ;
Samore, MH .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2005, 82 (04) :909-910
[3]  
Bergström J, 2000, SEMIN DIALYSIS, V13, P163, DOI 10.1046/j.1525-139x.2000.00044.x
[4]   INFLAMMATORY COMPOUNDS: NEUROPEPTIDE SUBSTANCE P AND CYTOKINES [J].
Castellani, M. L. ;
Felaco, P. ;
Pandolfi, F. ;
Salini, V. ;
De Amicis, D. ;
Vecchiet, J. ;
Tete, S. ;
Ciampoli, C. ;
Conti, F. ;
Cerulli, G. ;
Caraffa, A. ;
Antinolfi, P. ;
Cuccurullo, C. ;
Perrella, A. ;
Theoharides, T. C. ;
De Lutiis, M. A. ;
Kempuraj, D. ;
Shaik, Y. B. .
EUROPEAN JOURNAL OF INFLAMMATION, 2009, 7 (02) :63-69
[5]   MICROBIOLOGICAL AND BIOCHEMICAL EFFECTIVENESS OF AN ANTISEPTIC GEL ON THE BACTERIAL CONTAMINATION OF THE INNER SPACE OF DENTAL IMPLANTS: A 3-MONTH HUMAN LONGITUDINAL STUDY [J].
D'Ercole, S. ;
Tete, S. ;
Catamo, G. ;
Sammartino, G. ;
Femminella, B. ;
Tripodi, D. ;
Spoto, G. ;
Paolantonio, M. .
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2009, 22 (04) :1019-1026
[6]   IMMUNOMODULATORY ACTIVITY OF A PLANT EXTRACT CONTAINING HUMAN PAPILLOMAVIRUS 16-E7 PROTEIN IN HUMAN MONOCYTE-DERIVED DENDRITIC CELLS [J].
Di Bonito, P. ;
Grasso, F. ;
Mangino, G. ;
Massa, S. ;
Illiano, E. ;
Franconi, R. ;
Fanales-Belasio, E. ;
Falchi, M. ;
Affabris, E. ;
Giorgi, C. .
INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 2009, 22 (04) :967-978
[7]  
Di Pietro M, 2009, J BIOL REG HOMEOS AG, V23, P65
[8]   Multiple pathways for apoptotic nuclear fragmentation [J].
Dini, L ;
Coppola, S ;
Ruzittu, MT ;
Ghibelli, L .
EXPERIMENTAL CELL RESEARCH, 1996, 223 (02) :340-347
[9]   Carotid artery intima-media thickness correlates with oxidative stress in chronic haemodialysis patients with accelerated atherosclerosis [J].
Dursun, Belda ;
Dursun, Evrim ;
Suleymanlar, Gultekin ;
Ozben, Beste ;
Capraz, Irfan ;
Apaydin, Ali ;
Ozben, Tomris .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2008, 23 (05) :1697-1703
[10]   THE ROLE OF MOLECULAR CHAPERONES (HSPAs/HSP70s) IN ORAL HEALTH AND ORAL INFLAMMATORY DISEASES: A REVIEW [J].
Fabian, T. K. ;
Gotai, L. ;
Beck, A. ;
Fabian, G. ;
Fejerdy, P. .
EUROPEAN JOURNAL OF INFLAMMATION, 2009, 7 (02) :53-61