Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection

被引：5

作者：

Wang, Lin-Xu ^{[1
,3
]}

Mellon, Michael ^{[1
,2
]}

Bowder, Dane ^{[1
,3
]}

Quinn, Meghan ^{[1
,2
]}

Shea, Danielle ^{[1
,3
]}

Wood, Charles ^{[1
,3
]}

Xiang, Shi-Hua ^{[1
,2
]}

机构：

[1] Nebraska Ctr Virol, Lincoln, NE USA

[2] Sch Vet Med & Biomed Sci, Lincoln, NE USA

[3] Univ Nebraska Lincoln, Sch Biol Sci, Lincoln, NE 68583 USA

来源：

VIROLOGY | 2015年 / 475卷

关键词：

Surface display; E. coli K12 UT5600; scFv-VRC01; HIV-1; beta-Barrel domain; Autotransporter; VAGINAL LACTOBACILLUS STRAIN; COMMENSAL BACTERIA; FV; SECRETION; PROTEINS; EXPRESSION; NEUTRALIZATION; AUTODISPLAY; MICROBICIDE; RETROVIRUS;

D O I：

10.1016/j.virol.2014.11.018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to prevent HIV-1 infection. The VRC01 antibody mimics CD4-binding to gp120 and has broadly neutralization activities against HIV-1. We have designed a construct that can express the fusion peptide of the scFv-VRC01 antibody together with the autotransporter beta-barrel domain of IgAP gene from Neisseria gonorrhoeae, which enabled surface display of the antibody molecule. Our results indicate that the scFv-VRC01 antibody molecule was displayed on the surface of the bacteria as demonstrated by flow cytometry and immunofluorescence microscopy. The engineered bacteria can capture HIV-1 particles via surface-binding and inhibit HIV-1 infection in cell culture. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：179 / 186

页数：8

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