Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

被引：13

作者：

Faccio, G. ^{[1
]}

Bannwarth, M. B. ^{[2
]}

Schulenburg, C. ^{[1
]}

Steffen, V. ^{[3
]}

Jankowska, D. ^{[1
]}

Pohl, M. ^{[3
]}

Rossi, R. M. ^{[2
]}

Maniura-Weber, K. ^{[1
]}

Boesel, L. F. ^{[2
]}

Richter, M. ^{[1
]}

机构：

[1] Empa, Swiss Fed Labs Mat Sci & Technol, Lab Biointerfaces, Lerchenfeldstr 5, CH-9014 St Gallen, Switzerland

[2] Empa, Swiss Fed Labs Mat Sci & Technol, Lab Protect & Physiol, Lerchenfeldstr 5, CH-9014 St Gallen, Switzerland

[3] Forschungszentrum Julich GmbH, Biotechnol IBG 1, DE-52425 Julich, Germany

来源：

ANALYST | 2016年 / 141卷 / 13期

关键词：

FLUORESCENT NANOPARTICLES; ULTRASENSITIVE DETECTION; IMMOBILIZATION; PROTEINS; SURFACE; PROBE; CELLS;

D O I：

10.1039/c5an02573g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium-silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.

引用

页码：3982 / 3984

页数：3

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