Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing

被引：21

作者：

Aquino-Jarquin, Guillermo ^{[1
,2
]}

机构：

[1] Hosp Infantil Mexico Dr Federico Gomez, Lab Invest Genom Genet & Bioinformat, Dr Marquez 162, Ciudad De Mexico 06720, Mexico

[2] Univ Autonoma Metropolitana, Dept Ciencias Nat, Unidad Cuajimalpa, Ciudad De Mexico, Mexico

来源：

MOLECULAR GENETICS AND METABOLISM | 2021年 / 134卷 / 1-2期

关键词：

CRISPR; Cas9; Genome-editing; Off-target effects; Base editors; Prime editing; Anti-CRISPR proteins; DOUBLE-STRANDED BREAKS; DNA CLEAVAGE; CAS9; BASE; CRISPR-CAS9; SPECIFICITY; NUCLEASES; CHALLENGES; SYSTEMS; DAMAGE;

D O I：

10.1016/j.ymgme.2021.08.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

CRISPR/Cas9-based technology has revolutionized biomedical research by providing a high-fidelity gene-editing method, foreshadowing a significant impact on the therapeutics of many human genetic disorders previously considered untreatable. However, off-target events represent a critical hurdle before genome editing can be fully established in clinical practice. This mini-review recapitulates some recent advances for detecting and overcoming off-target effects mediated by the CRISPR/Cas9 system that could increase the likelihood of clinical success of the CRISPR-based approaches. (c) 2021 Elsevier Inc. All rights reserved.

引用

页码：77 / 86

页数：10

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