Fluorescent in situ hybridization in liver cell touch preparations from autopsy

被引：8
作者：
Adachi, PLG
Camparoto, ML
Sakamoto-Hojo, ET
Brassesco, MSA
Peres, LC
机构：
[1] USP, Fac Med Ribeirao Preto, Dept Pathol, BR-14049900 Ribeirao Preto, Brazil
[2] USP, Fac Med Ribeirao Preto Sci & Letters Ribeirao Pre, Dept Genet, BR-14049900 Ribeirao Preto, Brazil
[3] USP, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, BR-14049900 Ribeirao Preto, Brazil
来源：
PATHOLOGY RESEARCH AND PRACTICE | 2005年 / 201卷 / 01期
关键词：
autopsy; congenital anomalies; touch preparations; FISH;
D O I：
10.1016/j.prp.2004.11.004
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The objective of the present study was to develop a simplified and low-cost protocol for the investigation of congenital anomalies of chromosomal etiology by fluorescent in situ hybridization (FISH) using probes for chromosomes X, 18, 13/21 in liver cell touch preparations obtained from autopsies performed at the University Hospital of the Faculty of Medicine of Ribeirao Preto. Liver touch preparations were obtained from I I autopsy cases and fixed in 95% ethanol or methanol:acetic acid (3:1). The FISH technique was carried out according to the protocol of Pinkel [25] with modifications, using probes for chromosomes X, 18, 13/21. There was no significant difference in labeling intensity, quantity of nuclei, or number of signals present per nucleus between the materials fixed with the two fixatives. Similar results were obtained with different times of storage up to 14 months at -20 degrees C. We concluded that the use of touch preparations pretreated with acetic acid and fixed in 95% ethanol represents an efficient, practical, and low-cost method of cell preparation for FISH analysis. (c) 2004 Elsevier GmbH. All rights reserved.
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收藏
页码：41 / 47
页数：7
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