Detection and Analysis of Wnt Pathway Related lncRNAs Expression Profile in Lung Adenocarcinoma

被引：18

作者：

Chen, Jie ^{[1
]}

Hu, Lijuan ^{[2
]}

Chen, Jian ^{[2
]}

Pan, Qinshi ^{[2
]}

Ding, Hongyan ^{[2
]}

Xu, Gang ^{[2
]}

Zhu, Peiwu ^{[2
]}

Wen, Xiusu ^{[2
]}

Huang, Keta ^{[3
]}

Wang, Yumin ^{[2
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Intens Care Unit, Wenzhou, Peoples R China

[2] Wenzhou Med Univ, Affiliated Hosp 1, Dept Lab Med, Wenzhou 325000, Zhejiang, Peoples R China

[3] Wenzhou Med Univ, Affiliated Hosp 1, Dept Pathol, Wenzhou, Peoples R China

来源：

PATHOLOGY & ONCOLOGY RESEARCH | 2016年 / 22卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Lung adenocarcinoma; LncRNAs; Wnt pathway; Expression profile; SIGNALING PATHWAY; CANCER; CELLS;

D O I：

10.1007/s12253-016-0046-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Studies have shown that the expression profile of Wnt signaling pathway is very important in lung adenocarcinoma (LAD) and some lncRNAs can regulate the expression of key molecules of Wnt pathway. However, Wnt pathway related lncRNAs are not systematically analyzed and detected in lung adenocarcinoma. We used a high-throughput microarray to compare the lncRNA expression profiles in LAD and corresponding normal tissue (NT) samples. Several candidate Wnt pathway related lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. We found that 232 Wnt pathway related lncRNAs were obviously expressed (a parts per thousand yen2-fold change) in lung adenocarcinoma samples and 13 Wnt pathway related lncRNAs were aberrantly expressed in lung adenocarcinoma compared with matched histologically normal lung tissues by qPCR. Among these, RP11-181G12.2 and RP11-89 K21.1 were the most aberrantly expressed lncRNAs. Our study ascertained the expression of Wnt pathway related lncRNAs in lung adenocarcinoma. The results revealed that many Wnt pathway related lncRNAs were differentially expressed in lung adenocarcinoma tissues, suggesting that they may play a key role in tumor development.

引用

页码：609 / 615

页数：7

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