The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

被引：17

作者：

Valckx, Sara D. M. ^{[1
]}

De Bie, Jessie ^{[1
]}

Michiels, Ellen D. ^{[1
]}

Goovaerts, Ilse G. ^{[2
]}

Punjabi, Usha ^{[2
]}

Ramos-Ibeas, Priscila ^{[3
]}

Gutierrez-Adan, Alfonso ^{[3
]}

Bols, Peter E. ^{[1
]}

Leroy, Jo L. ^{[1
]}

机构：

[1] Univ Antwerp, Dept Vet Sci, B-2610 Antwerp, Belgium

[2] Univ Antwerp Hosp, Ctr Reprod Med, B-2650 Edegem, Belgium

[3] Inst Nacl Invest & Tecnol Agr & Alimentaria, INIA, Dept Reprod Anim, Madrid 28040, Spain

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2015年 / 30卷 / 02期

关键词：

embryo quality; follicular fluid; in-vitro maturation; IVF; oocyte developmental competence;

D O I：

10.1016/j.rbmo.2014.10.008

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or ` negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. (C) 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

引用

页码：203 / 207

页数：5

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