SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern

被引：310

作者：

McCallum, Matthew ^{[1
]}

Bassi, Jessica ^{[2
]}

De Marco, Anna ^{[2
]}

Chen, Alex ^{[3
]}

Walls, Alexandra C. ^{[1
]}

Di Iulio, Julia ^{[3
]}

Tortorici, M. Alejandra ^{[1
]}

Navarro, Mary-Jane ^{[1
]}

Silacci-Fregni, Chiara ^{[2
]}

Saliba, Christian ^{[2
]}

Sprouse, Kaitlin R. ^{[1
]}

Agostini, Maria ^{[3
]}

Pinto, Dora ^{[2
]}

Culap, Katja ^{[2
]}

Bianchi, Siro ^{[2
]}

Jaconi, Stefano ^{[2
]}

Cameroni, Elisabetta ^{[2
]}

Bowen, John E. ^{[1
]}

Tilles, Sasha W. ^{[4
]}

Pizzuto, Matteo Samuele ^{[2
]}

Guastalla, Sonja Bernasconi

Bona, Giovanni ^{[5
]}

Pellanda, Alessandra Franzetti ^{[5
]}

Garzoni, Christian ^{[6
]}

Van Voorhis, Wesley C. ^{[4
]}

Rosen, Laura E. ^{[3
]}

Snell, Gyorgy ^{[3
]}

Telenti, Amalio ^{[3
]}

Virgin, Herbert W. ^{[3
]}

Piccoli, Luca ^{[2
]}

Corti, Davide ^{[2
]}

Veesler, David ^{[1
]}

机构：

[1] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

[2] Humabs Biomed SA, CH-6500 Bellinzona, Switzerland

[3] Vir Biotechnol, San Francisco, CA 94158 USA

[4] Univ Washington, Ctr Emerging & Reemerging Infect Dis, Dept Med, Div Allergy & Infect Dis,Sch Med, Seattle, WA 98195 USA

[5] Clin Luganese Moncucco, Clin Res Unit, CH-6900 Lugano, Switzerland

[6] Clin Luganese Moncucco, Clin Internal Med & Infect Dis, CH-6900 Lugano, Switzerland

来源：

SCIENCE | 2021年 / 373卷 / 6555期

基金：

加拿大自然科学与工程研究理事会;

关键词：

RECEPTOR-BINDING DOMAIN; SPIKE; VALIDATION; MICROSCOPY; RESOLUTION; VISUALIZATION; SOFTWARE; SYSTEM; MODEL; ACE2;

D O I：

10.1126/science.abi7994

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel variant of concern (VOC) named CAL.20C (B.1.427/B.1.429), which was originally detected in California, carries spike glycoprotein mutations S13I in the signal peptide, W152C in the N-terminal domain (NTD), and L452R in the receptor-binding domain (RBD). Plasma from individuals vaccinated with a Wuhan-1 isolate-based messenger RNA vaccine or from convalescent individuals exhibited neutralizing titers that were reduced 2- to 3.5-fold against the B.1.427/B.1.429 variant relative to wild-type pseudoviruses. The L452R mutation reduced neutralizing activity in 14 of 34 RBD-specific monoclonal antibodies (mAbs). The S13I and W152C mutations resulted in total loss of neutralization for 10 of 10 NTD-specific mAbs because the NTD antigenic supersite was remodeled by a shift of the signal peptide cleavage site and the formation of a new disulfide bond, as revealed by mass spectrometry and structural studies.

引用

页码：648 / +

页数：41

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