Anti-hyperuricemic and nephroprotective effects of Smilax china L.

被引:85
作者
Chen, Lvyi [1 ]
Yin, Huafeng [1 ]
Lan, Zhou [2 ]
Ma, Shuwei [1 ]
Zhang, Chunfeng [1 ]
Yang, Zhonglin [1 ]
Li, Ping [1 ]
Lin, Baoqin [3 ]
机构
[1] China Pharmaceut Univ, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China
[2] China Pharmaceut Univ, Dept Pharmacol Chinese Mat Med, Nanjing 210009, Peoples R China
[3] Guangzhou Univ Chinese Med, Sch Tradit Chinese Med, Dept Pharmacol, Guangzhou 510006, Guangdong, Peoples R China
关键词
Smilax china L; Anti-hyperuricemic agents; Hyperuricemia; Uric acid; Nephroprotection; SERUM URIC-ACID; XANTHINE-OXIDASE; ALLOPURINOL;
D O I
10.1016/j.jep.2011.03.033
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Smilax china L, popularly known as "Jin Gang Ten", has been widely used as a traditional herbal medicine for the treatment of gout, rheumatoid arthritis and other diseases for a long time in China. Aim of study: The present study was carried out to investigate the effect of Smilax china L on hyperuricemia and renal dysfunction in induced hyperuricemic animals. Materials and methods: Five fractions (petroleum ether, chloroform, ethyl acetate, n-butanol and residual ethanol fraction) of Smilax china L were orally administered to potassium oxonate-induced hyperuricemic mice for three days. The xanthine oxidase inhibitory activities and modes of action of nine compounds isolated from ethyl acetate fraction (EAF) were then examined in vitro. Finally, different dosages of EAF were administered to 10% fructose-induced hyperuricemic rats. Results: EAF (250 mg/kg) exhibited stronger anti-hyperuricemic activity in hyperuricemic mice compared with the other four fractions. Caffeic acid, resveratrol, rutin and oxyresveratrol isolated from EAF showed different inhibitory activities on xanthine oxidase in vitro, with the IC50 values of 42.60, 37.53, 42.20 and 40.69 mu M, respectively, and exhibited competitive or mixed inhibitory actions. Moreover, EAF (125, 250 and 500 mg/kg) markedly reversed the serum uric acid level (p < 0.05, p < 0.01 and p < 0.001, respectively), fractional excretion of urate (p < 0.05, p < 0.01 and p < 0.01, respectively) and blood urea nitrogen (p < 0.05, p < 0.01 and p < 0.01, respectively) to their normal states, and prevented the renal damage against tubulointerstitial pathologies in hyperuricemic rats. Conclusion: These findings show that Smilax china L exhibits anti-hyperuricemic and nephroprotective activity in hyperuricemic animals. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:399 / 405
页数:7
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