Co-amplified quantitative real-time PCR: an objective comparison of H1N1 viral load between patients with mild, moderate, and severe disease

被引：0
作者：
Flatt, Andrew [1 ]
Grant, Paul R. [1 ]
Hudson, Lee D. [2 ]
Salt, Penny [2 ]
Down, Jim [3 ]
Singer, Mervyn [3 ]
Kidd, Mike [1 ]
机构：
[1] Univ Coll London Hosp, Dept Virol, London, England
[2] Univ Coll London Hosp, Dept Paediat, London, England
[3] Univ Coll London Hosp, Dept Anesthet & Crit Care, London, England
来源：
INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2011年 / 5卷
关键词：
Clinical severity; H1N1; quantitative polymerase chain reaction; swine influenza; viral load; INFLUENZA-VIRUS; RT-PCR; STORAGE; DNA; PATHOGENESIS;
D O I：
暂无
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Dual-primer PCR using quantitative measurement of swine 'flu and human pyruvate dehydrogenase (PDH) was performed on 100 nasopharyngeal swab (NPS) specimens to attain values of viral genome copy per human epithelial cell. Forty-two patients had accessible clinical details, and a study was made of the correlation of viral genome concentration with severity of clinical illness and biochemical markers of inflammation. No statistically significant correlations were found, although trends were observed with higher concentration values in mild illness. This may represent high numbers of virions prevented from migrating to the lower respiratory tract of patients with only mild disease.
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页码：290 / 293
页数：4
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