Unbalanced Vitreous Levels of Osteoprotegerin, RANKL, RANK, and TRAIL in Proliferative Diabetic Retinopathy

被引：10
作者：
Abu El-Asrar, Ahmed M. [1 ,2 ]
Ahmad, Ajmal [1 ]
Alam, Kaiser [1 ]
Bittoun, Emilie [3 ]
Siddiquei, Mohammad Mairaj [1 ]
Mohammad, Ghulam [1 ]
Mousa, Ahmed [1 ]
De Hertogh, Gert [3 ]
Opdenakker, Ghislain [4 ]
机构：
[1] King Saud Univ, Dept Ophthalmol, Coll Med, Riyadh, Saudi Arabia
[2] King Saud Univ, Coll Med, Dr Nasser Al Rashid Res Chair Ophthalmol, Riyadh, Saudi Arabia
[3] Univ Leuven, KU Leuven, Lab Histochem & Cytochem, Dept Pathol, Leuven, Belgium
[4] Univ Leuven, KU Leuven, Dept Microbiol & Immunol, Rega Inst Med Res, Leuven, Belgium
来源：
OCULAR IMMUNOLOGY AND INFLAMMATION | 2018年 / 26卷 / 08期
关键词：
Osteoprotegerin; proliferative diabetic retinopathy; RANK ligand; receptor activator of nuclear factor-kappa B (RANK); tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); vitreous; APOPTOSIS-INDUCING LIGAND; NF-KAPPA-B; ENDOTHELIAL GROWTH-FACTOR; RECEPTOR ACTIVATOR; INFLAMMATORY CYTOKINES; EXPRESSION; CELLS; ANGIOGENESIS; MARKER; SYSTEM;
D O I：
10.1080/09273948.2017.1343855
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Purpose: We investigated the expression of the proinflammatory and proangiogenic factor osteoprotegerin (OPG) and its ligands, receptor activator of nuclear factor-kappa B ligand (RANKL), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), and the receptor RANK in proliferative diabetic retinopathy (PDR). Materials and methods: Vitreous samples from PDR and nondiabetic control patients and epiretinal membranes from PDR patients were studied by enzyme-linked immunosorbent assay, immunohistochemistry, and Western blot analysis. Results: Vascular endothelial growth factor, OPG, and soluble RANK levels in vitreous samples from PDR patients were significantly higher than that in nondiabetic controls. Soluble TRAIL levels were significantly lower in PDR patients than that in nondiabetic control, whereas soluble RANKL levels did not differ significantly. RANKL, RANK, and TRAIL were expressed in vascular endothelial cells, myofibroblasts, and CD45-expressing leukocytes in PDR epiretinal membranes. Conclusions: Dysregulated expression of OPG/RANKL/RANK pathway and TRAIL might be related to inflammation and angiogenesis in PDR.
引用
收藏
页码：1248 / 1260
页数：13
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