A Multi-Channel Fiber Optic Photoluminescence System for Multiplex Biosensor Arrays

被引：1

作者：

Yi, Zhangjing ^{[1
,2
]}

Zhong, Zhong

Reardon, Kenneth F. ^{[3
]}

Wang, Weina ^{[1
,2
]}

Katragadda, Manasi ^{[1
,2
]}

Lear, Kevin L. ^{[1
,2
,3
]}

机构：

[1] Colorado State Univ, Dept Elect & Comp Engn, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA

[3] Colorado State Univ, Sch Biomed Engn, Ft Collins, CO 80523 USA

来源：

OPTICAL FIBERS AND SENSORS FOR MEDICAL DIAGNOSTICS AND TREATMENT APPLICATIONS X | 2010年 / 7559卷

关键词：

biosensor; fiber optic; photoluminescence; multiplex; multi-channel; optode; PHASE;

D O I：

10.1117/12.842900

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A multi-channel fiber optic photoluminescence system was developed for real time, in situ and continuous quantitative monitoring of chemical parameters in analytes, with the advantages of ease of use and low cost in manufacturing. The system sequentially excites 8 optodes and measures the resulting photoluminescence with a single photomultiplier. The oxygen sensitive phosphorescent dye or pH sensitive fluorescent dye in the optodes transduces the activity of an enzyme genetically engineered to detect a specific chemical species such as a chlorinated ethene. System operation is described and the relative signal is compared to measurements made with single channel system based on a commercial spectrometer.

引用

页数：6

共 11 条

[1] Optical dissolved oxygen lifetime sensor based on sol-gel immobilisation
Campbell, A
Uttamchandani, D
[J]. IEE PROCEEDINGS-SCIENCE MEASUREMENT AND TECHNOLOGY, 2004, 151 (04) : 291 - 297
[2] LUMINESCENCE QUENCHING MECHANISM FOR MICROHETEROGENEOUS SYSTEMS
CARRAWAY, ER
DEMAS, JN
DEGRAFF, BA
[J]. ANALYTICAL CHEMISTRY, 1991, 63 (04) : 332 - 336
[3] *EPA, 2009, MAX CONT LEV IN CHEM, P141
[4] Long-term continuous monitoring of dissolved oxygen in cell culture medium for perfused bioreactors using optical oxygen sensors
Gao, FG
Jeevarajan, AS
Anderson, MM
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (04) : 425 - 433
[5] GRATTON E, 1984, ANNU REV BIOPHYS BIO, V13, P105
[6] GRATTON E, 1993, Patent No. 0600633
[7] Fiber-optic oxygen sensor based on the fluorescence quenching of tris (5-acrylamido, 1,10 phenanthroline) ruthenium chloride
McNamara, KP
Li, XP
Stull, AD
Rosenzweig, Z
[J]. ANALYTICA CHIMICA ACTA, 1998, 361 (1-2) : 73 - 83
[8] Phosphorescence characteristics of ruthenium complex as an optical transducer for biosensors
Pieper, Sean B.
Mestas, Santano P.
Lear, Kevin L.
Zhong, Zhong
Reardon, Kenneth F.
[J]. APPLIED PHYSICS LETTERS, 2008, 92 (08)
[9] REARDON KF, 2010, ADV BIOCHEM ENG BIOT, P99
[10] A resazurin-based biosensor for organic pollutants
Tizzard, Aynsley C.
Bergsma, Joost H.
Lloyd-Jones, Gareth
[J]. BIOSENSORS & BIOELECTRONICS, 2006, 22 (05) : 759 - 763

← 1 2 →