Immobilization of DNA on Quartz Crystal Microbalance Sensor Modified with Self-Assembled Monolayer of Thiol Derivative

被引：6

作者：

Shibata, Hirobumi ^{[1
,2
]}

Nemoto, Junpei ^{[1
]}

Shiba, Shota ^{[1
]}

Yamane, Yusuke ^{[1
]}

Kawai, Gota ^{[3
]}

Hashimoto, Kazuaki ^{[1
]}

机构：

[1] Chiba Inst Technol, Fac Engn, 2-17-1 Tsudanuma, Narashino, Chiba 2750016, Japan

[2] Tokyo Univ Sci, Photocatalysis Int Res Ctr, 2641 Yamazaki, Noda, Chiba 2788510, Japan

[3] Chiba Inst Technol, Fac Adv Engn, 2-17-1 Tsudanuma, Narashino, Chiba 2750016, Japan

来源：

JOURNAL OF OLEO SCIENCE | 2020年 / 69卷 / 03期

关键词：

quartz crystal microbalance; self-assembled monolayer; DNA; thiol derivative; RESONANCE ENERGY-TRANSFER; SURFACE-PLASMON RESONANCE; BIOSENSOR; BINDING; FABRICATION; MECHANISMS; PEPTIDES;

D O I：

10.5650/jos.ess19317

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, we investigated the direct detection of DNA, without pretreatment, using a quartz crystal microbalance (QCM) sensor. This sensor is modified by a self-assembled monolayer of a thiol derivative that has an amino group as the terminal functional group. Contact angle values and the attenuated total reflectance Fourier transform infrared (ATR/FT-IR) spectra of the QCM sensors after immersion into an ethanol solution of thiol derivatives clearly showed that self-assembled monolayers of the derivatives were formed on the QCM sensors. Although QCM measurements using unmodified and carboxylic group-modified sensors could not detect DNA-Na salt, the sensor modified with amino groups could detect the DNA. This system can be used for the analysis of the interaction between DNA and DNA-binding proteins.

引用

页码：271 / 276

页数：6

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