Three-dimensional distribution of tyrosine hydroxylase, vasopressin and oxytocin neurones in the transparent postnatal mouse brain

被引:15
作者
Godefroy, D. [1 ,2 ]
Dominici, C. [1 ]
Hardin-Pouzet, H. [3 ]
Anouar, Y. [2 ]
Melik-Parsadaniantz, S. [1 ]
Rostene, W. [1 ]
Reaux-Le Goazigo, A. [1 ]
机构
[1] UPMC Univ Paris 06, Sorbonne Univ, Inst Vis, INSERM,CNRS,UMRS 968, Paris, France
[2] Normandie Univ, INSERM, UNIROUEN, U1239,DC2N,IRIB, Mont St Aignan, France
[3] UPMC Univ Paris 06, Neurosci Paris Seine Inst Biol Paris Seine, Sorbonne Univ, INSERM,CNRS, Paris, France
关键词
3D neuronal distribution; catecholamine; iDISCO; neuropeptide; transparent brain; DECARBOXYLASE-EXPRESSING NEURONS; NEUROPHYSIN-CONTAINING NEURONS; SUPRACHIASMATIC NUCLEUS; ONTOGENY; RAT; INNERVATION; ULTRAMICROSCOPY; VISUALIZATION; HYPOTHALAMUS; SYSTEM;
D O I
10.1111/jne.12551
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Over the years, advances in immunohistochemistry techniques have been a critical step in detecting and mapping neuromodulatory substances in the central nervous system. The better quality and specificity of primary antibodies, new staining procedures and the spectacular development of imaging technologies have allowed such progress. Very recently, new methods permitting tissue transparency have been successfully used on brain tissues. In the present study, we combined whole-mount immunostaining for tyrosine hydroxylase (TH), oxytocin (OXT) and arginine vasopressin (AVP), with the iDISCO+ clearing method, light-sheet microscopy and semi-automated counting of three-dimensionally-labelled neurones to obtain a (3D) distribution of these neuronal populations in a 5-day postnatal (P5) mouse brain. Segmentation procedure and 3D reconstruction allowed us, with high resolution, to map TH staining of the various catecholaminergic cell groups and their ascending and descending fibre pathways. We show that TH pathways are present in the whole P5 mouse brain, similar to that observed in the adult rat brain. We also provide new information on the postnatal distribution of OXT and AVP immunoreactive cells in the mouse hypothalamus, and show that, compared to AVP neurones, OXT neurones in the supraoptic (SON) and paraventricular (PVN) nuclei are not yet mature in the early postnatal period. 3D semi-automatic quantitative analysis of the PVN reveals that OXT cell bodies are more numerous than AVP neurones, although their immunoreactive soma have a volume half smaller. More AVP nerve fibres compared to OXT were observed in the PVN and the retrochiasmatic area. In conclusion, the results of the present study demonstrate the utility and the potency of imaging large brain tissues with clearing procedures coupled to novel 3D imaging technologies to study, localise and quantify neurotransmitter substances involved in brain and neuroendocrine functions.
引用
收藏
页数:10
相关论文
共 53 条
  • [1] Dynamical study of tyrosine hydroxylase expression and its correlation with vasopressin turnover in the magnocellular neurons of the supraoptico-posthypophysial system under long-term salt loading of adult rats
    Abramova, M
    Marsais, F
    Calas, A
    Thibault, J
    Ugrumov, M
    [J]. BRAIN RESEARCH, 2002, 925 (01) : 67 - 75
  • [2] Vasopressinergic neurons of the supraoptic nucleus in perinatal rats: reaction to osmotic stimulation and its regulation
    Abramova, M. A.
    Calas, A.
    Ugrumov, M.
    [J]. BRAIN STRUCTURE & FUNCTION, 2011, 215 (3-4) : 195 - 207
  • [3] ANDEN NILS-ERIK, 1965, LIFE SCI, V4, P1275, DOI 10.1016/0024-3205(65)90076-7
  • [4] Balan IS, 2000, J COMP NEUROL, V425, P167, DOI 10.1002/1096-9861(20000918)425:2<167::AID-CNE1>3.0.CO
  • [5] 2-K
  • [6] Tridimensional Visualization and Analysis of Early Human Development
    Belle, Morgane
    Godefroy, David
    Couly, Gerard
    Malone, Samuel A.
    Collier, Francis
    Giacobini, Paolo
    Chedotal, Alain
    [J]. CELL, 2017, 169 (01) : 161 - 173.e12
  • [7] A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System
    Belle, Morgane
    Godefroy, David
    Dominici, Chloe
    Heitz-Marchaland, Celine
    Zelina, Pavol
    Hellal, Farida
    Bradke, Frank
    Chedotal, Alain
    [J]. CELL REPORTS, 2014, 9 (04): : 1191 - 1201
  • [8] POSTNATAL-DEVELOPMENT OF THE SUPRACHIASMATIC NUCLEUS IN THE RAT - MORPHOFUNCTIONAL CHARACTERISTICS AND TIME-COURSE OF TYROSINE-HYDROXYLASE IMMUNOPOSITIVE FIBERS
    BELTRAMO, M
    CALAS, A
    CHERNIGOVSKAYA, E
    BORISOVA, N
    POLENOVA, O
    TILLET, Y
    THIBAULT, J
    UGRUMOV, M
    [J]. NEUROSCIENCE, 1994, 63 (02) : 603 - 610
  • [9] Bjorklund A, 1985, HDB POLYCYCLIC AROMA, P1
  • [10] Bjorklund A., 1984, HDB CHEM NEUROANATOM, P1