Induction of CEMIP in Chondrocytes by Inflammatory Cytokines: Underlying Mechanisms and Potential Involvement in Osteoarthritis

被引：21

作者：

Ohtsuki, Takashi ^{[1
]}

Hatipoglu, Omer F. ^{[1
]}

Asano, Keiichi ^{[2
]}

Inagaki, Junko ^{[3
]}

Nishida, Keiichiro ^{[4
]}

Hirohata, Satoshi ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol, Okayama 7008558, Japan

[2] Okayama Univ, Dept Mol Biol & Biochem, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan

[3] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Cell Chem, Okayama 7008558, Japan

[4] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaediac Surg, Kita Ku, 2-5-1 Shikata Cho, Okayama 7008558, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 09期

基金：

日本学术振兴会;

关键词：

cell migration-inducing hyaluronidase 1 (CEMIP); chondrocyte; hyaluronan; mechanical strain; nuclear factor kappa B (NF-kappa B); osteoarthritis; NF-KAPPA-B; OUMS-27 CHONDROSARCOMA CELLS; HYALURONIC-ACID; MYOCARDIAL-INFARCTION; EXPRESSION ANALYSIS; IN-VITRO; GENE; PROTEIN; ADAMTS1; FIBROBLASTS;

D O I：

10.3390/ijms21093140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In patients with osteoarthritis (OA), there is a decrease in both the concentration and molecular size of hyaluronan (HA) in the synovial fluid and cartilage. Cell migration-inducing hyaluronidase 1 (CEMIP), also known as hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID), was recently reported as an HA depolymerization-related molecule expressed in the cartilage of patients with OA. However, the underlying mechanism of CEMIP regulation is not well understood. We found that CEMIP expression was transiently increased by interleukine-1 beta (IL-1 beta) stimulation in chondrocytic cells. We also observed that ERK activation and NF-kappa B nuclear translocation were involved in the induction of CEMIP by IL-1 beta. In addition, both administration of HA and mechanical strain attenuated the CEMIP induction in IL-1 beta-stimulated chondrocytes. In conclusion, we clarified the regulatory mechanism of CEMIP in chondrocytes by inflammatory cytokines and suggested the potential involvement in osteoarthritis development.

引用

页数：15

共 56 条

[1] Interleukin-1 induced nuclear factor-B binds to a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 9 promoter in human chondrosarcoma cells [J].