Cell-free expression of functional receptor tyrosine kinases

被引：22

作者：

He, Wei ^{[1
]}

Scharadin, Tiffany M. ^{[2
]}

Saldana, Matthew ^{[3
]}

Gellner, Candice ^{[2
]}

Hoang-Phou, Steven ^{[1
]}

Takanishi, Christina ^{[2
]}

Hura, Gregory L. ^{[4
,5
]}

Tainer, John A. ^{[4
,6
]}

Carraway, Kermit L., III ^{[3
,7
]}

Henderson, Paul T. ^{[2
,7
]}

Coleman, Matthew A. ^{[1
,7
,8
]}

机构：

[1] Univ Calif Davis, Sch Med, Radiat Oncol, Sacramento, CA 95817 USA

[2] Univ Calif Davis, Sch Med, Internal Med, Div Hematol Oncol, Sacramento, CA 95817 USA

[3] Univ Calif Davis, Sch Med Biochem & Mol, Sacramento, CA 95817 USA

[4] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

[5] Univ Calif Santa Cruz, Chem & Biochem, Santa Cruz, CA 95064 USA

[6] Scripps Res Inst, La Jolla, CA 92037 USA

[7] Univ Calif Davis, Ctr Comprehens Canc, Sacramento, CA 95817 USA

[8] Lawrence Livermore Natl Lab, Livermore, CA USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

美国国家科学基金会;

关键词：

GROWTH-FACTOR RECEPTOR; X-RAY-SCATTERING; MEMBRANE-PROTEINS; ACTIVATION; ERBB2; SAXS;

D O I：

10.1038/srep12896

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.

引用

页数：8

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