Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase α1 subunit gene (atp1a1a.5)

被引：10

作者：

Blasiole, Brian

Canfield, Victor

Degrave, Agnes

Thisse, Christine

Thisse, Bernard

Rajarao, Johannes

Levenson, Robert ^{[1
]}

机构：

[1] Penn State Coll Med, Dept Pharmacol, Hershey, PA 17033 USA

[2] CNRS, INSERM, ULP, Inst Biol & Genet, Strasbourg, France

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 119卷

基金：

美国国家卫生研究院;

关键词：

zebrafish; Na; K-ATPase; alpha; 1; subunit; pronephros; ear;

D O I：

10.1016/S0925-4773(03)00118-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have identified and characterized cDNAs encoding a novel zebrafish Na, K-ATPase a subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish alpha 1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na, K-ATPase a1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb. (c) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：S211 / S214

页数：4

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