First cyclotide from Hybanthus (Violaceae)

被引：89

作者：

Broussalis, AM

Göransson, U

Coussio, JD

Ferraro, G

Martino, V

Claeson, P

机构：

[1] Uppsala Univ, Dept Med Chem, Div Pharmacognosy, Ctr Biomed, SE-75123 Uppsala, Sweden

[2] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Farmacognosia, CONICET,IQUIMEFA, RA-1053 Buenos Aires, DF, Argentina

来源：

PHYTOCHEMISTRY | 2001年 / 58卷 / 01期

关键词：

Hybanthus parviflorus (Mutis ex L. F.) Baill; Violaceae; violetilla; isolation; primary structure; cyclotides; macrocyclic polypeptides; hypa A; LCQ;

D O I：

10.1016/S0031-9422(01)00173-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypa A, a novel macrocyclic polypeptide containing 30 amino acid residues, has been isolated from the n-butanol extract of the Argentine plant Hybanthus parviflorus. The sequence, cyclo-(SCVYIPCTITALLGCSCKNKVCYNGIPCAE), was determined by automated Edman degradation, quantitative amino acid analysis and nanospray MS/MS2. Three intramolecular disulfide bridges stabilize the cyclic peptide backbone of hypa A. Using these structural features to classify the peptide as a cyclotide, we extended the distribution of that substance class to a new genus. and now propose a uniform nomenclature for cyclotides. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：47 / 51

页数：5

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