Anti-biofilm activity of A22 ((S-3,4-dichlorobenzyl) isothiourea hydrochloride) against Pseudomonas aeruginosa: Influence on biofilm formation, motility and bioadhesion

被引：19

作者：

Bonez, Pauline Cordenonsi ^{[1
]}

Rossi, Grazielle Guidolin ^{[1
]}

Bandeira, Jardel Rodrigo ^{[2
]}

Ramos, Andiara Prates ^{[2
]}

Mizdal, Caren Rigon ^{[1
]}

Agertt, Vanessa Albertina ^{[1
]}

Segatto Dalla Nora, Eloisa Salete ^{[1
]}

de Souza, Marcia Ebling ^{[3
]}

dos Santos Alves, Camilla Fillippi ^{[1
]}

dos Santos, Fallon Siqueira ^{[1
]}

Gundel, Andre ^{[1
]}

Vaucher, Rodrigo de Almeida ^{[4
]}

Vianna Santos, Roberto Christ ^{[1
]}

Anraku de Campos, Marli Matiko ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Grad Program Pharmaceut Sci, Ave Roraima 1000, BR-97105900 Santa Maria, RS, Brazil

[2] Ctr Univ Franciscano, Cell Culture Lab, Rua Andradas 1614, BR-97010032 Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Oral, Ave Roraima 1000, BR-97105900 Santa Maria, RS, Brazil

[4] Univ Fed Pelotas, Microbiol Res Lab, Rua Gomes Carneiro 1, BR-96010610 Pelotas, Brazil

来源：

MICROBIAL PATHOGENESIS | 2017年 / 111卷

关键词：

A22; Adhesion; Bacterial motility; Biofilms; Pseudomonas aeruginosa; Surfaces; ACTIN-LIKE PROTEIN; BACTERIAL BIOFILMS; MREB; COMPOUND; DIVERSE; IMPACT;

D O I：

10.1016/j.micpath.2017.08.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bacterial biofilms are involved in various medical infections and for this reason it is of great importance understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Different approaches have been used for preventing biofilm related infections in health care settings, such as use of surface coatings agents in medical implants. In this context, is necessary to explore new compounds with anti-biofilm activity. Thus, this study evaluated for the first time the action of A22 against biofilms of Pseudomonas aeruginosa PAO1 strain and multi-resistant clinical isolates on biotic and abiotic surfaces. A22 acts as inhibitor of the MreB protein of the bacterial cell wall, causing the rods to change shape to the coccoid form. In this work, A22 at subinhibitory concentrations was able to prevent biofilm formation, and atomic force microscopy images showed that A22 was highly effective in inhibiting adhesion on polyethylene surfaces. Pseudomonas aeruginosa PAO1 exhibited a strong ability to adhere to HeLa cells, and A22 inhibited the aggregation after 4 h of exposure. Swarming and twitching motilities were significantly altered by A22 at subinhibitory concentrations. Thus, by changing the shape of the bacterial cell, many properties can be affected, such as motility, surface adhesion and biofilm formation. This work presents A22 as a promising novel antibacterial or surface coating agent of medical materials. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：6 / 13

页数：8

共 38 条

[1] A22 Disrupts the Bacterial Actin Cytoskeleton by Directly Binding and Inducing a Low-Affinity State in MreB [J].