A novel method for calculating mean erythrocyte age using erythrocyte creatine

被引：10

作者：

Kameyama, Masashi ^{[1
]}

Koga, Masafumi ^{[2
]}

Okumiya, Toshika ^{[3
]}

机构：

[1] Tokyo Metropolitan Geriatr Hosp & Inst Gerontol, Dept Diagnost Radiol, Tokyo, Japan

[2] Hakuhokai Cent Hosp, Dept Internal Med, Amagasaki, Hyogo, Japan

[3] Kumamoto Univ, Dept Biomed Lab Sci, Fac Hlth Sci, Kumamoto, Japan

来源：

AGING-US | 2020年 / 12卷 / 09期

关键词：

creatine; erythrocyte; average RBC age; lifespan; hemolysis; HEMOGLOBIN;

D O I：

10.18632/aging.103193

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Estimating the lifespan of erythrocytes is useful for the differential diagnosis of anemia. However, measuring the lifespan of erythrocytes was very difficult; therefore, it was seldom measured. Erythrocyte creatine (EC) decreases reflecting erythrocyte age. We developed a method to obtain mean erythrocyte age (M-RBC) from EC. We reanalyzed the previously published data from 21 patients with hemolytic anemia, which included EC and the half-life of Cr-51. M-RBC and log(e) EC showed excellent significant linearity (r = -0.9475, p < 0.001), proving that it could be treated as a mono-exponential relationship within the studied range (EC: 1.45 - 11.76 mu mol/g Hb). We established an equation to obtain M-RBC (days) from EC (mu mol/g Hb): M-RBC = -22.84log(e) EC + 65.83. This equation allowed calculation of M-RBC based on EC which has practical applications such as the diagnosis of anemia.

引用

页码：8702 / 8709

页数：8

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