The determination of busulphan in dissolution samples by flow injection analysis

被引：4

作者：

Dow, AD ^{[1
]}

Finlay, G ^{[1
]}

Bloomfield, MS ^{[1
]}

机构：

[1] Glaxo Wellcome Res & Dev Ltd, Pharmaceut Dev, Ware SG12 0DP, Herts, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 19卷 / 3-4期

关键词：

flow injection analysis; automation; busulphan; Myleran (R); dissolution;

D O I：

10.1016/S0731-7085(98)00252-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A robust colourimetric dow injection analysis (FIA) procedure is described for the determination of busulphan in dissolution samples of a 2 mg tablet formulation. The sample solution is injected directly into a reagent stream containing 4-(4-nitrobenzyl)pyridine/potassium hydrogen phthalate. An on-line heating stage allows the formation of a coloured pyrridinium salt species, which following stabilisation is detected spectrophotometrically at 570 nm. The method has been fully validated and is linear over the concentration range 0.004-0.024 mg of busulphan ml(-1). The method can also been applied to uniformity of content and bulk assay testing. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：549 / 554

页数：6