WSL9Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice

被引：8

作者：

Zhu, Xingjie ^{[1
]}

Mou, Changling ^{[1
]}

Zhang, Fulin ^{[1
]}

Huang, Yunshuai ^{[1
]}

Yang, Chunyan ^{[1
]}

Ji, Jingli ^{[1
]}

Liu, Xi ^{[1
]}

Cao, Penghui ^{[1
]}

Thanhliem Nguyen ^{[1
,2
]}

Lan, Jie ^{[1
]}

Zhou, Chunlei ^{[1
,2
,3
]}

Liu, Shijia ^{[1
]}

Jiang, Ling ^{[1
]}

Wan, Jianmin ^{[1
,3
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Peoples R China

[2] Quynhon Univ, Dept Biol & Agr Engn, Quynhon 590000, Binhdinh, Vietnam

[3] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

来源：

RICE | 2020年 / 13卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Oryza sativa; Chloroplasts; RNA splicing; PENTATRICOPEPTIDE REPEAT PROTEIN; DNA-BINDING; PLANT-MITOCHONDRIA; RNA-POLYMERASES; NUCLEAR GENE; BIOGENESIS; PLASTIDS; RESTRICTION; IDENTIFICATION; SEQUENCE;

D O I：

10.1186/s12284-020-00407-2

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Background The plant chloroplast is essential for photosynthesis and other cellular processes, but an understanding of the biological mechanisms of plant chloroplast development are incomplete. Results A new temperature-sensitivewhite stripe leaf 9(wsl9)rice mutant is described. The mutant develops white stripes during early leaf development, but becomes green after the three-leaf stage under field conditions. Thewsl9mutant was albinic when grown at low temperature. Gene mapping of theWSL9locus, together with complementation tests indicated thatWSL9encodes a novel protein with an HNH domain.WSL9was expressed in various tissues. Under low temperature, thewsl9mutation caused defects in splicing ofrpl2, but increased the editing efficiency ofrpoB.Expression levels of plastid genome-encoded genes, which are transcribed by plastid-coded RNA polymerase (PEP), chloroplast development genes and photosynthesis-related genes were altered in thewsl9mutant. Conclusion WSL9encodes an HNH endonuclease domain-containing protein that is essential for early chloroplast development. Our study provides opportunities for further research on regulatory mechanisms of chloroplast development in rice.

引用

页数：13

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