Bioluminescence and its application in the monitoring of antimicrobial photodynamic therapy

被引：24

作者：

Alves, Eliana ^{[1
,2
]}

Costa, Liliana ^{[1
,2
]}

Cunha, Angela ^{[1
,2
]}

Faustino, Maria Amparo F. ^{[3
,4
]}

Neves, Maria Graca P. M. S. ^{[3
,4
]}

Almeida, Adelaide ^{[1
,2
]}

机构：

[1] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal

[3] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal

[4] Univ Aveiro, QOPNA, P-3810193 Aveiro, Portugal

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2011年 / 92卷 / 06期

关键词：

Bioluminescence; Biotechnology; Antimicrobial photodynamic therapy; Biomedical research; Environmental microbiology; Tetrapyrrolic macrocycles; Drug monitoring; REPORTER BACTERIOPHAGE A511LUXAB; RESISTANT STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; BACTERIAL BIOLUMINESCENCE; XENORHABDUS-LUMINESCENS; SINGLET OXYGEN; PORPHYRIN DERIVATIVES; LUX GENES; STREPTOCOCCUS-PNEUMONIAE; SUPEROXIDE-DISMUTASE;

D O I：

10.1007/s00253-011-3639-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Light output from bioluminescent microorganisms is a highly sensitive reporter of their metabolic activity and therefore can be used to monitor in real time the effects of antimicrobials. Antimicrobial photodynamic therapy (aPDT) is receiving considerable attention for its potentialities as a new antimicrobial treatment modality. This therapy combines oxygen, a nontoxic photoactive photosensitizer, and visible light to generate reactive oxygen species (singlet oxygen and free radicals) that efficiently destroy microorganisms. To monitor this photoinactivation process, faster methods are required instead of laborious conventional plating and overnight incubation procedures. The bioluminescence method is a very interesting approach to achieve this goal. This review covers recent developments on the use of microbial bioluminescence in aPDT in the clinical and environmental areas.

引用

页码：1115 / 1128

页数：14

共 151 条

[41]

Desjardin DE, 2005, FUNGAL DIVERS, V18, P9

[42] Visualizing fungal infections in living mice using bioluminescent pathogenic Candida albicans strains transformed with the firefly luciferase gene [J].